Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.
AuthorsGiepmans BN, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH
JournalNat Methods
PubMed ID16179920
'The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron microscope. Here we report the application ... More
Multiplexed hybridization detection of quantum dot-conjugated DNA sequences using surface plasmon enhanced fluorescence microscopy and spectrometry.
AuthorsRobelek R, Niu L, Schmid EL, Knoll W
JournalAnal Chem
PubMed ID15481968
'In this study, the general suitability of quantum dot (QD)-DNA conjugates for the surface plasmon enhanced fluorescence spectroscopy technique is demonstrated. Furthermore, the QD-DNA system is transferred to the platform of surface plasmon enhanced fluorescence microscopy. Using this technique together with a microarray format, in which the sensor-bound single-stranded catcher ... More
Near-complete suppression of quantum dot blinking in ambient conditions.
AuthorsHohng S, Ha T
JournalJ Am Chem Soc
PubMed ID14759174
Continuous distribution of emission states from single CdSe/ZnS quantum dots.
AuthorsZhang K, Chang H, Fu A, Alivisatos AP, Yang H,
JournalNano Lett
PubMed ID16608295
The photoluminescence dynamics of colloidal CdSe/ZnS/streptavidin quantum dots were studied using time-resolved single-molecule spectroscopy. Statistical tests of the photon-counting data suggested that the simple
Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution.
Water-soluble quantum dots (qdots) are now being used in life sciences research to take advantage of their bright, easily excited fluorescence and high photostability. Although the frequent erratic blinking and substantial dark (never radiant) fractions that occur in all available qdots may interfere with many applications, these properties of individual ... More