Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.
AuthorsGiepmans BN, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH
JournalNat Methods
PubMed ID16179920
'The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron microscope. Here we report the application ... More
Multiplexed hybridization detection of quantum dot-conjugated DNA sequences using surface plasmon enhanced fluorescence microscopy and spectrometry.
AuthorsRobelek R, Niu L, Schmid EL, Knoll W
JournalAnal Chem
PubMed ID15481968
'In this study, the general suitability of quantum dot (QD)-DNA conjugates for the surface plasmon enhanced fluorescence spectroscopy technique is demonstrated. Furthermore, the QD-DNA system is transferred to the platform of surface plasmon enhanced fluorescence microscopy. Using this technique together with a microarray format, in which the sensor-bound single-stranded catcher ... More
Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia.
AuthorsZhu L, Ang S, Liu WT
JournalAppl Environ Microbiol
PubMed ID14711692
Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia. ... More
Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection.
AuthorsNess JM, Akhtar RS, Latham CB, Roth KA
JournalJ Histochem Cytochem
PubMed ID12871979
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple ... More
Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells.
In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity ... More
Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots.
AuthorsWu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, Peale F, Bruchez MP
JournalNat Biotechnol
PubMed ID12459735
Semiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and ... More