Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers.
AuthorsHadrup SR, Bakker AH, Shu CJ, Andersen RS, van Veluw J, Hombrink P, Castermans E, Thor Straten P, Blank C, Haanen JB, Heemskerk MH, Schumacher TN,
JournalNat Methods
PubMed ID19543285
Abstract:'The use of fluorescently labeled major histocompatibility complex multimers has become an essential technique for analyzing disease- and therapy-induced T-cell ... More
Single-molecule quantum-dot fluorescence resonance energy transfer.
AuthorsHohng S, Ha T
JournalChemphyschem
PubMed ID15884082
Abstract:'Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof ... More
Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes.
AuthorsTelford WG
JournalCytometry A
PubMed ID15351984
Abstract:'INTRODUCTION: Violet laser diodes have become common and reliable laser sources for benchtop flow cytometers. While these lasers are very ... More
The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy.
AuthorsWu Y, Campos SK, Lopez GP, Ozbun MA, Sklar LA, Buranda T
JournalAnal Biochem
PubMed ID17397793
Abstract:'The use of fluorescence calibration beads has been the hallmark of quantitative flow cytometry. It has enabled the direct comparison ... More
Quantum dot self-assembly for protein detection with sub-picomolar sensitivity.
AuthorsSoman CP, Giorgio TD,
JournalLangmuir
PubMed ID18335969
Abstract:'A novel approach to sensitive and rapid antigen detection is described. In the presence of a specific antigen, quantum dot-antibody ... More
Separation of bioconjugated quantum dots using capillary electrophoresis.
AuthorsVicente G, Colón LA,
JournalAnal Chem
PubMed ID18278946
Abstract:'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD ... More
Nanometer distance measurements between multicolor quantum dots.
Abstract:'Quantum dot dimers made of short double-stranded DNA molecules labeled with different color quantum dots at each end were imaged ... More
Quantum dot mediated imaging of atherosclerosis.
AuthorsJayagopal A, Su YR, Blakemore JL, Linton MF, Fazio S, Haselton FR,
JournalNanotechnology
PubMed ID19420562
Abstract:'The progression of atherosclerosis is associated with leukocyte infiltration within lesions. We describe a technique for the ex vivo imaging ... More
Method for multiplex cellular detection of mRNAs using quantum dot fluorescent in situ hybridization.
Abstract:'The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ ... More
Photodynamics of a single quantum dot: fluorescence activation, enhancement, intermittency, and decay.
AuthorsLee SF, Osborne MA
JournalJ Am Chem Soc
PubMed ID17608479
Inflammation in fetal sheep from intra-amniotic injection of Ureaplasma parvum.
AuthorsCollins JJ, Kallapur SG, Knox CL, Nitsos I, Polglase GR, Pillow JJ, Kuypers E, Newnham JP, Jobe AH, Kramer BW,
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID20935228
Abstract:Bronchopulmonary dysplasia is associated with chorioamnionitis and fetal lung inflammation. Ureaplasma species are the bacteria most frequently isolated from chorioamnionitis. ... More
Suppression of quantum dot blinking in DTT-doped polymer films.
AuthorsAntelman J, Ebenstein Y, Dertinger T, Michalet X, Weiss S,
JournalJ Phys Chem C Nanomater Interfaces
PubMed ID20161096
Abstract:In this report we evaluate the emission properties of single quantum dots embedded in a thin, thiol containing polymer film. ... More
Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.
AuthorsAndrews NL, Lidke KA, Pfeiffer JR, Burns AR, Wilson BS, Oliver JM, Lidke DS,
JournalNat Cell Biol
PubMed ID18641640
Abstract:The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI ... More
Comparison of detection and signal amplification methods for DNA microarrays.
AuthorsVora GJ, Meador CE, Anderson GP, Taitt CR,
JournalMol Cell Probes
PubMed ID18675897
Abstract:One of the factors limiting the use of DNA microarray technology for the detection of pathogenic organisms from clinical and ... More
Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging.
AuthorsLe Gac S, Vermes I, van den Berg A
JournalNano Lett
PubMed ID16967992
Abstract:Quantum dots (Qdots) are nanoparticles exhibiting fluorescent properties that can be used for cell staining. We present here the development ... More
Spectroscopic characterization of streptavidin functionalized quantum dots.
AuthorsWu Y, Lopez GP, Sklar LA, Buranda T
JournalAnal Biochem
PubMed ID17368555
Abstract:The spectroscopic properties of quantum dots can be strongly influenced by the conditions of their synthesis. In this work, we ... More
Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.
AuthorsFerrara DE, Weiss D, Carnell PH, Vito RP, Vega D, Gao X, Nie S, Taylor WR
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID16223849
Abstract:Traditional imaging with one-photon confocal microscopy and organic fluorophores poses several challenges for the visualization of vascular tissue, including autofluorescence, ... More
Multispectral imaging of clinically relevant cellular targets in tonsil and lymphoid tissue using semiconductor quantum dots.
AuthorsFountaine TJ, Wincovitch SM, Geho DH, Garfield SH, Pittaluga S
JournalMod Pathol
PubMed ID16778828
Abstract:Determination of the expression and spatial distribution of molecular epitopes, or antigens, in patient tissue specimens has substantially improved the ... More