Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies.
AuthorsAlanio C, Lemaitre F, Law HK, Hasan M, Albert ML,
JournalBlood
PubMed ID20200354
'The number of antigen-specific naive CD8(+) T cells is believed to be important in the shaping of adaptive immune responses, and is predictive for the magnitude of priming responses in mouse models. Because of extremely low precursor frequencies, knowledge about these cells comes from indirect techniques and estimations. Here, we ... More
TNF-a induces upregulation of EGFR expression and signaling in human colonic myofibroblasts.
AuthorsYoo J, Rodriguez Perez CE, Nie W, Edwards RA, Sinnett-Smith J, Rozengurt E,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID22301110
The myofibroblast has recently been identified as an important mediator of tumor necrosis factor-a (TNF-a)-associated colitis and cancer, but the mechanism(s) involved remains incompletely understood. Recent evidence suggests that TNF-a is a central regulator of multiple inflammatory signaling cascades. One important target of TNF-a may be the signaling pathway downstream ... More
GTPgammaS microtubules mimic the growing microtubule end structure recognized by end-binding proteins (EBs).
AuthorsMaurer SP, Bieling P, Cope J, Hoenger A, Surrey T,
JournalProc Natl Acad Sci U S A
PubMed ID21368119
Microtubule plus-end-tracking proteins (+TIPs) localize to growing microtubule plus ends to regulate a multitude of essential microtubule functions. End-binding proteins (EBs) form the core of this network by recognizing a distinct structural feature transiently existing in an extended region at growing microtubule ends and by recruiting other +TIPs to this ... More
Apical surface expression of aspartic protease Plasmepsin 4, a potential transmission-blocking target of the plasmodium ookinete.
To invade its definitive host, the mosquito, the malaria parasite must cross the midgut peritrophic matrix that is composed of chitin cross-linked by chitin-binding proteins and then develop into an oocyst on the midgut basal lamina. Previous evidence indicates that Plasmodium ookinete-secreted chitinase is important in midgut invasion. The mechanistic ... More
Semiautomated multiplexed quantum dot-based in situ hybridization and spectral deconvolution.
AuthorsByers RJ, Di Vizio D, O'connell F, Tholouli E, Levenson RM, Gossage K, Gossard K, Twomey D, Yang Y, Benedettini E, Rose J, Ligon KL, Finn SP, Golub TR, Loda M,
JournalJ Mol Diagn
PubMed ID17251332
Gene expression profiling has identified several potentially useful gene signatures for predicting outcome or for selecting targeted therapy. However, these signatures have been developed in fresh or frozen tissue, and there is a need to apply them to routinely processed samples. Here, we demonstrate the feasibility of a potentially high-throughput ... More