On-chip aptamer-based sandwich assay for thrombin detection employing magnetic beads and quantum dots.
AuthorsTennico YH, Hutanu D, Koesdjojo MT, Bartel CM, Remcho VT,
JournalAnal Chem
PubMed ID20545301
In this paper, we report the development of an on-chip aptamer-based fluorescence assay for protein detection and quantification based on sandwich ELISA principles. Thrombin was selected as a model analyte to validate the assay design, which involves two DNA thrombin aptamers recognizing two different epitopes of the protein. Aptamer-functionalized magnetic ... More