Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Actual product may vary
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Invitrogen™

Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂

使用荧光 Quant-iT RNA 试剂和测定试剂盒可实现对高丰度 RNA 样品的高选择性和准确定量。相对于双链 DNA,这些试剂盒对 RNA 具有高度选择性,其提供的材料足够以96孔微孔板规格进行至少2000次测定。
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货号数量检测定量范围
Q102131,000 assaysRNA 定量,宽范围20 至 1000 ng
Q331401 kitRNA 定量5 至 100 ng
Q332251000 assaysRNA 定量,扩展范围200 至 10000 ng
Q328841 mLRNA 定量,高灵敏度5 至 100 ng
货号 Q10213
价格(CNY)
6,173.00
Each
添加至购物车
数量:
1,000 assays
检测:
RNA 定量,宽范围
定量范围:
20 至 1000 ng
价格(CNY)
6,173.00
Each
添加至购物车
使用 Quant-iT RNA 定量试剂盒可以实现简单、准确的 RNA 定量,该试剂盒具有宽范围 (BR) 和扩展范围 (XR) 配置且含有独立的高灵敏度 (HS) 试剂。相较于 dsDNA,这些 Quant-iT RNA 定量试剂盒对 RNA 具有高度选择性,可用于实现基于荧光的线性 RNA 检测范围。
Quant-iT RNA 试剂和测定试剂盒
Quant-iT RNA 试剂和测定试剂盒使 RNA 定量分析变得轻松、准确。该试剂盒提供浓缩的检测试剂、稀释缓冲液以及预稀释的 RNA 标准品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 5-100 ng RNA 的动态范围。

Quant-iT RNA BR(宽范围)测定试剂盒
Quant-iT RNA BR(宽范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。原装 Quant-iT RNA 试剂盒具有很高的灵敏度,而 Quant-iT RNA 宽范围试剂盒适用于 RNA 浓度较高的微阵列样品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 20-1000 ng RNA 的动态范围。

Quant-iT RNA XR(扩展范围)测定试剂盒
Quant-iT RNA XR(扩展范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。该检测试剂对于 RNA 具有高度选择性,不能定量 DNA、蛋白或游离核苷酸。该测定试剂盒设计用于准确检测初始浓度为 10 ng/µL 至 10,000 ng/µL 的 RNA 样品(取决于样品体积),线性检测范围为 200–10,000 ng。

每次测定,只需使用提供的缓冲液对试剂进行稀释,加入样品(1 µL 至 20 µL 的任意体积均可接受),然后就可使用基于荧光的酶标仪读取浓度值。对常见污染物,如盐、游离核苷酸、溶剂、洗涤剂和蛋白质等,有着很好的耐受性。

仅供科研使用。不可用于诊断程序。
规格
检测RNA 定量,宽范围
激发/发射644/673
适用于(设备)微孔板读数仪
反应次数1000次反应(200 μL 测定体积)
产品线Quant-iT
定量范围20 至 1000 ng
数量1,000 assays
运输条件室温
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

为什么我用Qubit Assay检测时会得到负的荧光值?

负的荧光值物理上是不可能的。它是由于软件自动扣除背景信号而造成的假象。这意味着你的荧光计检测到背景信号并将其扣除从而牺牲了真实数据。务必做一个仅有缓冲液的对照并评估信号的类型。你可能需要换用另外一块板。

我有一个Quant-iT RNA检测试剂盒,而想将它用于Qubit荧光计。可以吗?

可以,使用手册中有关于此应用的说明。

这些Quant-iT RNA试剂盒适用于siRNA和microRNA吗?

不适用,它们不能精确定量那么小的片段。Quant-iT microRNA检测试剂盒和Qubit microRNA检测试剂盒非常适合此类应用。

Quant-iT RNA检测试剂可以用于定量地高辛标记的RNA吗?

可以的,该标记物不会干扰信号。

Quant-iT RNA试剂盒的线性范围是多少?

线性范围如下:

•Quant-iT RiboGreen RNA检测试剂:1 ng/mL至1 µg/mL
•Quant-iT RNA BR检测试剂:20–1,000 ng
•Quant-iT RNA HS检测试剂:5–100 ng

引用和文献 (6)

引用和文献
Abstract
[Possible Involvement of Genes Related to Lysosomal Storage Disorders in the Pathogenesis of Parkinson's Disease].
Authors:Rudenok MM, Alieva AK, Nikolaev MA, Kolacheva AA, Ugryumov MV, Pchelina SN, Slominsky PA, Shadrina MI
Journal:
PubMed ID:30895950
Parkinson's disease (PD) characterized with slow continuous degeneration of dopaminergic neurons in the substantia nigra is one of the most common neurodegenerative diseases, but its etiology and pathogenesis are not fully understood. The pathogenesis of PD involves the impairment of lysosomal autophagy, which also contributes to lysosomal storage disorders (LSDs). ... More
Expression analysis of genes involved in mitochondrial biogenesis in mice with MPTP-induced model of Parkinson's disease.
Authors:Rudenok MM, Alieva AK, Starovatykh JS, Nesterov MS, Stanishevskaya VA, Kolacheva AA, Ugryumov MV, Slominsky PA, Shadrina MI
Journal:Mol Genet Metab Rep
PubMed ID:32280590
'The mitochondrion is an extremely important organelle that performs various functions in the cell: e.g. energy production, regulation of respiration processes and maintenance of calcium homeostasis. Disruption of the biogenesis and functioning of this organelle can lead to cell damage and cell death. Mitochondrial dysfunction has been shown to possibly ... More
PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites.
Authors:Ipe J, Collins KS, Hao Y, Gao H, Bhatia P, Gaedigk A, Liu Y, Skaar TC
Journal:Front Genet
PubMed ID:29963077
Next-generation sequencing (NGS) studies have identified large numbers of genetic variants that are predicted to alter miRNA-mRNA interactions. We developed a novel high-throughput bioassay, PASSPORT-seq, that can functionally test in parallel 100s of these variants in miRNA binding sites (mirSNPs). The results are highly reproducible across both technical and biological ... More
RNA editing blood biomarkers for predicting mood alterations in HCV patients.
Authors:Salvetat N, Van der Laan S, Vire B, Chimienti F, Cleophax S, Bronowicki JP, Doffoel M, Bourlière M, Schwan R, Lang JP, Pujol JF, Weissmann D
Journal:J Neurovirol
PubMed ID:31332697
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-a and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies such as inflammatory disorders, depression and suicide. Interestingly, IFN-a induces ... More
Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells.
Authors:Elsemüller AK, Tomalla V, Gärtner U, Troidl K, Jeratsch S, Graumann J, Baal N, Hackstein H, Lasch M, Deindl E, Preissner KT, Fischer S
Journal:FASEB J
PubMed ID:30702929
Tissue-resident mast cells (MCs) are well known for their role in inflammatory responses and allergic and anaphylactic reactions, but they also contribute to processes of arterial remodeling. Although ribosomes and cytosolic RNAs are located around secretory granules in mature MCs, their functional role in MC responses remains unexplored. Previous studies ... More