Qdot™ 655 生物素偶联试剂盒
Qdot™ 655 生物素偶联试剂盒
Invitrogen™

Qdot™ 655 生物素偶联试剂盒

生物素标记的 Qdot™ 655 纳米晶体可用于检测链霉素亲和素探针或使用链霉素亲和素桥与链霉素亲和素标记的分子或其他生物素化分子形成非共价偶联物。产品以 250 µL 2 µM 溶液的形式提供,包括了解更多信息
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货号数量
Q10321MP1 kit
货号 Q10321MP
价格(CNY)
12,343.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
12,343.00
Each
添加至购物车
生物素标记的 Qdot™ 655 纳米晶体可用于检测链霉素亲和素探针或使用链霉素亲和素桥与链霉素亲和素标记的分子或其他生物素化分子形成非共价偶联物。产品以 250 µL 2 µM 溶液的形式提供,包括 30 mL Qdot™ 孵育缓冲液。
仅供科研使用。不可用于诊断程序。
规格
产品类型生物素 Qdot 偶联试剂盒
数量1 kit
运输条件室温
产品线Qdot
Unit SizeEach
内容与储存
冷藏储存 (2–8°C)。

常见问题解答 (FAQ)

有什么好办法可以从ITK Qdot纳米晶中去除白色沉淀?

在3,000 rpm下离心ITK Qdot纳米晶3-5分钟,可以从上清中去除白色沉淀,之后就可以直接使用上清了。

我在ITK Qdot纳米晶中发现白色沉淀,是否应该引起注意?

有机ITK Qdot纳米晶中有一定几率会析出白色沉淀。ITK Qdot纳米晶有时候会含有呈白色沉淀状的杂质。

为什么我的Qdot纳米晶似乎在闪烁?

闪烁是量子点固有的性质;实际上,所有的单一发冷光的分子都会闪烁,包括有机染料。由于其亮度和光稳定性,Qdot纳米晶的闪烁显得更加明显。激发能量越高,Qdot纳米晶闪烁甚至更快。使用Beta-巯基乙醇可以减少闪烁。

Qdot纳米晶在封固样品前荧光很亮,但现在我只能观测到少量的荧光,为什么会发生荧光衰减?

选择合适的封固液对保持Qdot纳米晶荧光性能至关重要。根据我们的研究,Qdot纳米晶适合用下列封固液:

•HistoMount封固液(货号00-8030),非常适合长期保存
•Cytoseal 60封固液
•Clarion封固液
•大多数基于聚乙烯醇的封固液(有限储存时间,<数周)
•基于水的封固液(有限储存时间,<1周)
•至多50%的甘油 (有限储存时间,<1周)

注:我们不推荐ProLong封固液与Qdot 纳米晶一同使用,因其会导致Qdot发生荧光淬灭。

为什么不能冷冻Qdot纳米晶溶液?

冷冻会导致产品聚集。一旦聚集后就不能重新分散到溶液内。

引用和文献 (4)

引用和文献
Abstract
Separation of bioconjugated quantum dots using capillary electrophoresis.
Authors:Vicente G, Colón LA,
Journal:Anal Chem
PubMed ID:18278946
'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to ... More
Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay.
Authors:Hildebrandt N, Charbonnière LJ, Beck M, Ziessel RF, Löhmannsröben HG
Journal:Angew Chem Int Ed Engl
PubMed ID:16247812
Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy.
Authors:Charbonnière LJ, Hildebrandt N, Ziessel RF, Löhmannsröben HG,
Journal:J Am Chem Soc
PubMed ID:17002375
A time-resolved fluoro-immunoassay (TR-FIA) format is presented based on resonance energy transfer from visible emitting lanthanide complexes of europium and terbium, as energy donors, to semiconductor CdSe/ZnS core/shell nanocrystals (quantum dots, QD), as energy acceptors. The spatial proximity of the donor-acceptor pairs is obtained through the biological recognition process of ... More
Single particle quantum dot imaging achieves ultrasensitive detection capabilities for Western immunoblot analysis.
Authors:Scholl B, Liu HY, Long BR, McCarty OJ, O'Hare T, Druker BJ, Vu TQ,
Journal:ACS Nano
PubMed ID:19453141
Substantially improved detection methods are needed to detect fractionated protein samples present at trace concentrations in complex, heterogeneous tissue and biofluid samples. Here we describe a modification of traditional Western immunoblotting using a technique to count quantum-dot-tagged proteins on optically transparent PVDF membranes. Counts of quantum-dot-tagged proteins on immunoblots achieved ... More