Qdot™ 655 Biotin Conjugate Kit

Citations & References (4)

Invitrogen™

Qdot™ 655 Biotin Conjugate Kit

The biotin-labeled Qdot™ 655 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules orRead more

Catalog Number	Quantity
Q10321MP	1 kit

Catalog number Q10321MP

Price (CNY)

12,343.00

Add to cart

Quantity:

1 kit

Price (CNY)

12,343.00

Add to cart

The biotin-labeled Qdot™ 655 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules or with other biotinylated molecules using a streptavidin bridge. The product is provided as 250 μL of a 2 μM solution and includes 30 mL of Qdot™ incubation buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeBiotin Qdot Conjugation Kit

Quantity1 kit

Shipping ConditionRoom Temperature

Product LineQdot

Unit SizeEach

Contents & Storage

Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why can't I freeze my Qdot nanocrystal solution?

Freezing will cause the product to aggregate. The Qdot nanocrystals cannot be dispersed into solution after aggregation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 655 Biotin Conjugate Kit FAQs

Citations & References (4)

Citations & References

Abstract

Separation of bioconjugated quantum dots using capillary electrophoresis.

Authors:Vicente G, Colón LA,

Journal:Anal Chem

PubMed ID:18278946

'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to ... More

Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay.

Authors:Hildebrandt N, Charbonnière LJ, Beck M, Ziessel RF, Löhmannsröben HG

Journal:Angew Chem Int Ed Engl

PubMed ID:16247812

Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy.

Authors:Charbonnière LJ, Hildebrandt N, Ziessel RF, Löhmannsröben HG,

Journal:J Am Chem Soc

PubMed ID:17002375

A time-resolved fluoro-immunoassay (TR-FIA) format is presented based on resonance energy transfer from visible emitting lanthanide complexes of europium and terbium, as energy donors, to semiconductor CdSe/ZnS core/shell nanocrystals (quantum dots, QD), as energy acceptors. The spatial proximity of the donor-acceptor pairs is obtained through the biological recognition process of ... More

Single particle quantum dot imaging achieves ultrasensitive detection capabilities for Western immunoblot analysis.

Authors:Scholl B, Liu HY, Long BR, McCarty OJ, O'Hare T, Druker BJ, Vu TQ,

Journal:ACS Nano

PubMed ID:19453141

Substantially improved detection methods are needed to detect fractionated protein samples present at trace concentrations in complex, heterogeneous tissue and biofluid samples. Here we describe a modification of traditional Western immunoblotting using a technique to count quantum-dot-tagged proteins on optically transparent PVDF membranes. Counts of quantum-dot-tagged proteins on immunoblots achieved ... More