Droplet microfluidic technology for single-cell high-throughput screening.
AuthorsBrouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML,
JournalProc Natl Acad Sci U S A
PubMed ID19617544
'We present a droplet-based microfluidic technology that enables high-throughput screening of single mammalian cells. This integrated platform allows for the encapsulation of single cells and reagents in independent aqueous microdroplets (1 pL to 10 nL volumes) dispersed in an immiscible carrier oil and enables the digital manipulation of these reactors ... More
The short splice variant of the gamma 2 subunit acts as an external modulator of GABA(A) receptor function.
AuthorsBoileau AJ, Pearce RA, Czajkowski C,
JournalJ Neurosci
PubMed ID20371809
GABA(A) receptors (GABA(A)Rs) regulate the majority of fast inhibition in the mammalian brain and are the target for multiple drug types, including sleep aids, anti-anxiety medication, anesthetics, alcohol, and neurosteroids. A variety of subunits, including the highly distributed gamma2, allow for pharmacologic and kinetic differences in particular brain regions. The ... More
3D tracking of single nanoparticles and quantum dots in living cells by out-of-focus imaging with diffraction pattern recognition.
Authors
JournalSci Rep
PubMed ID26526410
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.