A technical note on quantum dots for multi-color fluorescence in situ hybridization.
AuthorsMüller S, Cremer M, Neusser M, Grasser F, Cremer T,
JournalCytogenet Genome Res
PubMed ID19556786
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors. We established a FISH protocol for the simultaneous visualization of up to 6 different DNA probes differentially labeled with Qdots and with conventional organic fluorochromes. Using ... More