A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease.
AuthorsCummings RT, Salowe SP, Cunningham BR, Wiltsie J, Park YW, Sonatore LM, Wisniewski D, Douglas CM, Hermes JD, Scolnick EM
JournalProc Natl Acad Sci U S A
PubMed ID11997440
'A fluorescence resonance energy transfer assay has been developed for monitoring Bacillus anthracis lethal factor (LF) protease activity. A fluorogenic 16-mer peptide based on the known LF protease substrate MEK1 was synthesized and found to be cleaved by the enzyme at the anticipated site. Extension of this work to a ... More