AuthorsKeren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
JournalNat Cell Biol
PubMed ID19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo.
AuthorsGibson VB, Benson RA, Bryson KJ, McInnes IB, Rush CM, Grassia G, Maffia P, Jenkinson EJ, White AJ, Anderson G, Brewer JM, Garside P,
JournalBlood
PubMed ID22271449
'In vivo imaging has revolutionized understanding of the spatiotemporal complexity that subserves the generation of successful effector and regulatory immune responses. Until now, invasive surgery has been required for microscopic access to lymph nodes (LNs), making repeated imaging of the same animal impractical and potentially affecting lymphocyte behavior. To allow ... More
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalNano Lett
PubMed ID17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More
Granule exocytosis is required for platelet spreading: differential sorting of a-granules expressing VAMP-7.
AuthorsPeters CG, Michelson AD, Flaumenhaft R,
JournalBlood
PubMed ID22589474
There has been recent controversy as to whether platelet a-granules represent a single granule population or are composed of different subpopulations that serve discrete functions. To address this question, we evaluated the localization of vesicle-associated membrane proteins (VAMPs) in spread platelets to determine whether platelets actively sort a specific subpopulation ... More
Surface coatings determine cytotoxicity and irritation potential of quantum dot nanoparticles in epidermal keratinocytes.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalJ Invest Dermatol
PubMed ID16902417
Quantum dot (QD) nanoparticles have potential applications in nanomedicine as drug delivery vectors and diagnostic agents, but the skin toxicity and irritation potential of QDs are unknown. Human epidermal keratinocytes (HEKs) were used to assess if QDs with different surface coatings would cause differential effects on HEK cytotoxicity, proinflammatory cytokine ... More
Penetration of intact skin by quantum dots with diverse physicochemical properties.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalToxicol Sci
PubMed ID16443688
Skin is the largest organ of the body and is a potential route of exposure to engineered nanomaterials, but the permeability of the skin to these nanomaterials is unknown. We selected commercially available quantum dots (QD) of two core/shell sizes and shapes and three different surface coatings to determine if ... More
Towards Stable Trapping of Single Macromolecules in Solution.
Authors
JournalProc SPIE Int Soc Opt Eng
PubMed ID23814448
Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses.
Authors
JournalNat Immunol
PubMed ID31611698
Impedance analysis of adherent cells after in situ electroporation-mediated delivery of bioactive proteins, DNA and nanoparticles in µL-volumes.