A quantum dot-aptamer beacon using a DNA intercalating dye as the FRET reporter: application to label-free thrombin detection.
Authors:Chi CW, Lao YH, Li YS, Chen LC,
Journal:Biosens Bioelectron
PubMed ID:21306887
'A new quantum dot (QD)-aptamer (apt) beacon that acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3(B), is demonstrated with label-free thrombin detection. The beacon, denoted as QD-apt:B, is constructed by (1) coupling of a single-stranded thrombin aptamer to Qdot 565 via EDC/Sulfo-NHS chemistry and (2) staining the duplex ... More
In vivo real-time, multicolor, quantum dot lymphatic imaging.
Authors:Kosaka N, Ogawa M, Sato N, Choyke PL, Kobayashi H,
Journal:J Invest Dermatol
PubMed ID:19536144
'The lymphatic network is complex and difficult to visualize in real-time in vivo. Moreover, the direction of flow within lymphatic networks is often unpredictable especially in areas with well-developed ' ... More
Simultaneous multicolor imaging of five different lymphatic basins using quantum dots.
Authors:Kobayashi H, Hama Y, Koyama Y, Barrett T, Regino CA, Urano Y, Choyke PL
Journal:Nano Lett
PubMed ID:17530812
Quantum dots can be used to perform multicolor images with high fluorescent intensity and are of a nanosize suitable for lymphatic imaging via direct interstitial injection. Here simultaneous multicolor in vivo wavelength-resolved spectral fluorescence lymphangiography is shown using five quantum dots with similar physical sizes but different emission spectra. This ... More
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
Journal:Nano Lett
PubMed ID:17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More
In vivo skin penetration of quantum dot nanoparticles in the murine model: the effect of UVR.
Ultraviolet radiation (UVR) has widespread effects on the biology and integrity of the skin barrier. Research on the mechanisms that drive these changes, as well as their effect on skin barrier function, has been ongoing since the 1980s. However, no studies have examined the impact of UVR on nanoparticle skin ... More