In vivo real-time, multicolor, quantum dot lymphatic imaging.
Authors:Kosaka N, Ogawa M, Sato N, Choyke PL, Kobayashi H,
Journal:J Invest Dermatol
PubMed ID:19536144
'The lymphatic network is complex and difficult to visualize in real-time in vivo. Moreover, the direction of flow within lymphatic networks is often unpredictable especially in areas with well-developed ' ... More
microPET-based biodistribution of quantum dots in living mice.
This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. (64)Cu-Labeled 800- or 525-nm emission wavelength QD (21- or 12-nm diameter), with or without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting ... More
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.
Authors:Shalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, Macbeath G, Yang EG, Park H,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20080678
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies ... More
Optimized linkage and quenching strategies for quantum dot molecular beacons.
Authors:Cady NC, Strickland AD, Batt CA
Journal:Mol Cell Probes
PubMed ID:17084590
Quantum dot (QD) molecular beacons were explored for sequence-specific DNA detection. The effectiveness of multiple linkage strategies and fluorescence quenchers were compared in hybridization-based assays. To compare linkage strategies, covalent amide linkage and streptavidin-biotin binding were used to link semiconductor QDs to molecular beacon DNA. Amide-linked beacons showed a 57% ... More