Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Authors:Nikiforov TT, Beechem JM
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More
Targeted quantum dot conjugates for siRNA delivery.
Authors:Derfus AM, Chen AA, Min DH, Ruoslahti E, Bhatia SN
Journal:Bioconjug Chem
PubMed ID:17630789
'Treatment of human diseases such as cancer generally involves the sequential use of diagnostic tools and therapeutic modalities. Multifunctional platforms combining therapeutic and diagnostic imaging functions in a single vehicle promise to change this paradigm. in particular, nanoparticle-based multifunctional platforms offer the potential to improve the pharmacokinetics of drug formulations, ... More
Specific and covalent labeling of a membrane protein with organic fluorochromes and quantum dots.
Authors:Bonasio R, Carman CV, Kim E, Sage PT, Love KR, Mempel TR, Springer TA, von Andrian UH
Journal:Proc Natl Acad Sci U S A
PubMed ID:17785425
'The real-time observation of protein dynamics in living cells and organisms is of fundamental importance for understanding biological processes. Most approaches to labeling proteins exploit noncovalent interactions, unsuitable to long-term studies, or genetic fusion to naturally occurring fluorescent proteins that often have unsatisfactory optical properties. Here we used the fungal ... More
Optimizing a waveguide-based sandwich immunoassay for tumor biomarkers: evaluating fluorescent labels and functional surfaces.
Authors:Mukundan H, Xie H, Anderson AS, Grace WK, Shively JE, Swanson BI,
Journal:Bioconjug Chem
PubMed ID:19173652
'The sensor team at the Los Alamos National Laboratory has developed a waveguide-based optical biosensor for the detection of biomarkers associated with disease. We have previously demonstrated the application of this technology to the sensitive detection of carcinoembryonic antigen in serum and nipple aspirate fluid from breast cancer patients. In ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Authors:Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
Journal:ACS Nano
PubMed ID:23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More