Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
AuthorsLiu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
JournalACS Nano
PubMed ID23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Copper-free click chemistry for highly luminescent quantum dot conjugates: application to in vivo metabolic imaging.
AuthorsBernardin A, Cazet A, Guyon L, Delannoy P, Vinet F, Bonnaffé D, Texier I,
JournalBioconjug Chem
PubMed ID20222737
Quantum dots (QD) are inorganic nanocrystals with outstanding optical properties, specially suited for biological imaging applications. Their attachment to biomolecules in mild aqueous conditions for the design of bioconjugates is therefore highly desirable. 1,3-dipolar [3 + 2] cycloaddition between azides and terminal alkynes ( ... More
Real-time imaging of astrocyte response to quantum dots: in vivo screening model system for biocompatibility of nanoparticles.
AuthorsMaysinger D, Behrendt M, Lalancette-Hébert M, Kriz J
JournalNano Lett
PubMed ID17638392
Astrocytes are the principle macroglial brain cells. They are activated by different stressors and brain injuries. Quantum dots (QDs) can cause oxidative stress. This study shows a real-time imaging of primary cortical cultures and assessment of QD-induced activation of astrocytes in the brains of transgenic mice with the luciferase gene ... More
The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line.
AuthorsClift MJ, Rothen-Rutishauser B, Brown DM, Duffin R, Donaldson K, Proudfoot L, Guy K, Stone V,
JournalToxicol Appl Pharmacol
PubMed ID18708083
This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also ... More
Qdot nanobarcodes for multiplexed gene expression analysis.
AuthorsEastman PS, Ruan W, Doctolero M, Nuttall R, de Feo G, Park JS, Chu JS, Cooke P, Gray JW, Li S, Chen FF
JournalNano Lett
PubMed ID16683851
We report a quantum dot (Qdot) nanobarcode-based microbead random array platform for accurate and reproducible gene expression profiling in a high-throughput and multiplexed format. Four different sizes of Qdots, with emissions at 525, 545, 565, and 585 nm are mixed with a polymer and coated onto the 8-mum-diameter magnetic microbeads ... More