'Drug screening is often limited to cell-free assays involving purified enzymes, but it is arguably best applied against systems that represent disease states or complex physiological cellular networks. Here, we describe a high-content, cell-based drug discovery platform based on phosphospecific flow cytometry, or phosphoflow, that enabled screening for inhibitors against ... More
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling.
AuthorsKrutzik PO, Nolan GP
JournalNat Methods
PubMed ID16628206
'Flow cytometry allows high-content, multiparameter analysis of single cells, making it a promising tool for drug discovery and profiling of intracellular signaling. To add high-throughput capacity to flow cytometry, we developed a cell-based multiplexing technique called fluorescent cell barcoding (FCB). In FCB, each sample is labeled with a different signature, ... More
Characterization of the functional binding properties of antibody conjugated quantum dots.
AuthorsPathak S, Davidson MC, Silva GA
JournalNano Lett
PubMed ID17536868
'Antibody conjugated quantum dots are an emerging technology for high-resolution labeling of biological systems. In this work we determined the number of functional antibodies (i.e., antibodies that are sterically available for functional binding to target proteins) conjugated to semiconductor quantum dots. This is critical for the interpretation of biological data ... More
Quantification of human urinary exosomes by nanoparticle tracking analysis.
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA from the glomerulus and all sections of the nephron and represent a reservoir for biomarker discovery. Current methods for the identification and quantification of urinary exosomes are time consuming and only semi-quantitative. Nanoparticle tracking ... More