Visible-to-near IR quantum dot-based hypermulticolor high-content screening of herbal medicines for the efficacy monitoring of hair growth promotion and hair loss inhibition.
AuthorsKim MJ, Lim C, Lee JY, Im KR, Yoon KS, Song JM,
JournalJ Biomol Screen
PubMed ID23190736
'There is a growing interest in alopecia prevention strategies, as the number of alopecia patients is increasing. We examine the efficacy of herbal medicine for hair growth promotion/hair loss inhibition in two cell lines via Western blot and high-content screening (HCS). Nine herbal extracts were obtained from three different herbal ... More
Separation of bioconjugated quantum dots using capillary electrophoresis.
AuthorsVicente G, Colón LA,
JournalAnal Chem
PubMed ID18278946
'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to ... More
Quantum-dot-based electrochemical immunoassay for high-throughput screening of the prostate-specific antigen.
AuthorsWang J, Liu G, Wu H, Lin Y,
JournalSmall
PubMed ID18081131
In this Communication, we demonstrate an electrochemical high-throughput sensing platform for simple, sensitive detection of PSA based on QD labels. This sensing platform uses a microplate for immunoreactions and disposable screen-printed electrodes (SPE) as an electrochemical transducer for stripping analysis of metal ions released from the captured QD labels in ... More
Rapid up-regulation and granule-independent transport of perforin to the immunological synapse define a novel mechanism of antigen-specific CD8+ T cell cytotoxic activity.
CTL are endowed with the ability to eliminate pathogens through perforin-mediated cytotoxic activity. The mechanism for perforin-mediated Ag-specific killing has been solely attributed to cytotoxic granule exocytosis from activated CD8(+) T cells. In this study, we redefine this mechanism, demonstrating that virus-specific CD8(+) T cells rapidly up-regulate perforin in response ... More
Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies.
AuthorsZelman-Femiak M, Wang K, Gromova K, Knaus P, Harms G,
JournalBiotechniques
PubMed ID20701592
Here we describe a labeling technique for the covalent linkage of quantum dots to transmembrane receptors for single-molecule tracking. Our method combines the acyl carrier protein (ACP) technique with coenzyme A (CoA)-functionalized quantum dots to covalently attach quantum dots to ACP fusions of receptor proteins. The advantages of this approach ... More
Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry.
AuthorsXing Y, Chaudry Q, Shen C, Kong KY, Zhau HE, Chung LW, Petros JA, O'Regan RM, Yezhelyev MV, Simons JW, Wang MD, Nie S
JournalNat Protoc
PubMed ID17546006
Bioconjugated quantum dots (QDs) provide a new class of biological labels for evaluating biomolecular signatures (biomarkers) on intact cells and tissue specimens. In particular, the use of multicolor QD probes in immunohistochemistry is considered one of the most important and clinically relevant applications. At present, however, clinical applications of QD-based ... More
Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms.
AuthorsChalmers NI, Palmer RJ, Du-Thumm L, Sullivan R, Shi W, Kolenbrander PE
JournalAppl Environ Microbiol
PubMed ID17114321
Oral biofilms are multispecies communities, and in their nascent stages of development, numerous bacterial species engage in interspecies interactions. Better insight into the spatial relationship between different species and how species diversity increases over time can guide our understanding of the role of interspecies interactions in the development of the ... More