In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dots.
AuthorsGonda K, Watanabe TM, Ohuchi N, Higuchi H,
JournalJ Biol Chem
PubMed ID19917603
Changes in membrane morphology and membrane protein dynamics based on its fluidity are critical for cancer metastasis. However, this subject has remained unclear, because the spatial precision of previous in vivo imaging has been limited to the micrometer level and single molecule imaging is impossible. Here, we have imaged the ... More
Surface antigen profiling of colorectal cancer using antibody microarrays with fluorescence multiplexing.
A procedure is described for the disaggregation of colorectal cancers (CRC) and normal intestinal mucosal tissues to produce suspensions of viable single cells, which are then captured on customized antibody microarrays recognising 122 different surface antigens (DotScan CRC microarray). Cell binding patterns recorded by optical scanning of microarrays provide a ... More