Glycoprotein D actively induces rapid internalization of two nectin-1 isoforms during herpes simplex virus entry.
AuthorsStiles KM, Krummenacher C,
JournalVirology
PubMed ID20089288
'Entry of herpes simplex virus (HSV) occurs either by fusion at the plasma membrane or by endocytosis and fusion with an endosome. Binding of glycoprotein D (gD) to a receptor such as nectin-1 is essential in both cases. We show that virion gD triggered the rapid down-regulation of nectin-1 with ... More
Alzheimer disease macrophages shuttle amyloid-beta from neurons to vessels, contributing to amyloid angiopathy.
AuthorsZaghi J, Goldenson B, Inayathullah M, Lossinsky AS, Masoumi A, Avagyan H, Mahanian M, Bernas M, Weinand M, Rosenthal MJ, Espinosa-Jeffrey A, de Vellis J, Teplow DB, Fiala M,
JournalActa Neuropathol
PubMed ID19139910
'Neuronal accumulation of oligomeric amyloid-beta (Alphabeta) is considered the proximal cause of neuronal demise in Alzheimer disease (AD) patients. Blood-borne macrophages might reduce Abeta stress to neurons by immigration into the brain and phagocytosis of Alphabeta. We tested migration and export across a blood-brain barrier model, and phagocytosis and clearance ... More
Multiplexing of pathway-specific beta-lactamase reporter gene assays by optical coding with Qtracker nanocrystals.
AuthorsMachleidt T, Whitney P, Bi K,
JournalJ Biomol Screen
PubMed ID19502565
'Reporter assays are widely used in research and drug discovery for analysis of signaling pathways in a cell-based format. Traditionally, reporter gene assays are run in a single-parameter mode, interrogating only 1 pathway per sample. To enable more complex assay formats for pathway analysis, the authors developed a multiplexed reporter ... More
'ABSTRACT: BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, fat and muscle cells and are being investigated for their utility in cell-based transplantation therapy. Yet, adequate methods to track transplanted MSCs in vivo are limited, precluding functional studies. Quantum Dots (QDs) offer ... More
Engineering the brain tumor microenvironment enhances the efficacy of dendritic cell vaccination: implications for clinical trial design.
AuthorsMineharu Y, King GD, Muhammad AK, Bannykh S, Kroeger KM, Liu C, Lowenstein PR, Castro MG,
JournalClin Cancer Res
PubMed ID21632862
'Glioblastoma multiforme (GBM) is a deadly primary brain tumor. Clinical trials for GBM using dendritic cell (DC) vaccination resulted in antitumor immune responses. Herein, we tested the hypothesis that combining in situ (intratumoral) Ad-Flt3L/Ad-TK-mediated gene therapy with DC vaccination would increase therapeutic efficacy and antitumor immunity. We first assessed the ... More
Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer.
AuthorsZheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, Edil BH, Mizuma M, Sharma R, Le DT, Anders RA, Illei PB, Van Eyk JE, Maitra A, Laheru D, Jaffee EM,
JournalPLoS One
PubMed ID21572519
'The aggressiveness of pancreatic ductal adenocarcinoma (PDA) is characterized by its high metastatic potential and lack of effective therapies, which is the result of a lack of understanding of the mechanisms involved in promoting PDA metastases. We identified Annexin A2 (ANXA2), a member of the Annexin family of calcium-dependent phospholipid ... More
In vivo study of the effects of peptide-conjugated near-infrared fluorescent quantum dots on the tumorigenic and lymphatic metastatic capacities of squamous cell carcinoma cell line Tca8113 and U14.
AuthorsLi ZG, Yang K, Cao YA, Zheng G, Sun DP, Zhao C, Yang J,
JournalInt J Mol Sci
PubMed ID20480027
Quantum dots (QDs) have great potential in non-invasive monitoring and imaging of tumor cells in vivo, but it is unknown if QDs affect their tumorigenesis and metastasis. Here, we applied peptide-conjugated near-infrared fluorescent QDs (NIRF-QDs) to label the squamous cell carcinoma cells Tca8113 and U14. We tested the proliferation and ... More
Observation of individual microtubule motor steps in living cells with endocytosed quantum dots.
AuthorsNan X, Sims PA, Chen P, Xie XS
JournalJ Phys Chem B
PubMed ID16375416
We report the observation of individual steps taken by motor proteins in living cells by following movements of endocytic vesicles that contain quantum dots (QDs) with a fast camera. The brightness and photostability of quantum dots allow us to record motor displacement traces with 300 micros time resolution and 1.5 ... More
MEF2C enhances dopaminergic neuron differentiation of human embryonic stem cells in a parkinsonian rat model.
AuthorsCho EG, Zaremba JD, McKercher SR, Talantova M, Tu S, Masliah E, Chan SF, Nakanishi N, Terskikh A, Lipton SA,
JournalPLoS One
PubMed ID21901155
Human embryonic stem cells (hESCs) can potentially differentiate into any cell type, including dopaminergic neurons to treat Parkinson's disease (PD), but hyperproliferation and tumor formation must be avoided. Accordingly, we use myocyte enhancer factor 2C (MEF2C) as a neurogenic and anti-apoptotic transcription factor to generate neurons from hESC-derived neural stem/progenitor ... More
Identification and characterization of a novel multipotent sub-population of Sca-1? cardiac progenitor cells for myocardial regeneration.
AuthorsTakamiya M, Haider KH, Ashraf M,
JournalPLoS One
PubMed ID21980409
METHODS AND RESULTS: The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and low Sca-1 (CSL1; 37.1%; CSL2; ... More
Segmental versican expression in the trabecular meshwork and involvement in outflow facility.
Versican is a large proteoglycan with numerous chondroitin sulfate (CS) glycosaminoglycan (GAG) side chains attached. To assess versican's potential contributions to aqueous humor outflow resistance, its segmental distribution in the trabecular meshwork (TM) and the effect on outflow facility of silencing the versican gene were evaluated. Fluorescent quantum dots (Qdots) ... More
Generation and labeling of murine bone marrow-derived dendritic cells with Qdot nanocrystals for tracking studies.
AuthorsMuccioli M, Pate M, Omosebi O, Benencia F,
JournalJ Vis Exp
PubMed ID21673641
Dendritic cells (DCs) are professional antigen presenting cells (APCs) found in peripheral tissues and in immunological organs such as thymus, bone marrow, spleen, lymph nodes and Peyer's patches. DCs present in peripheral tissues sample the organism for the presence of antigens, which they take up, process and present in their ... More
Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion.
AuthorsBrentnall TA, Lai LA, Coleman J, Bronner MP, Pan S, Chen R,
JournalPLoS One
PubMed ID22291919
Cancer-associated fibroblasts, comprised of activated fibroblasts or myofibroblasts, are found in the stroma surrounding solid tumors. These myofibroblasts promote invasion and metastasis of cancer cells. Mechanisms regulating the activation of the fibroblasts and the initiation of invasive tumorigenesis are of great interest. Upregulation of the cytoskeletal protein, palladin, has been ... More
Conversion of vascular endothelial cells into multipotent stem-like cells.
Mesenchymal stem cells can give rise to several cell types, but varying results depending on isolation methods and tissue source have led to controversies about their usefulness in clinical medicine. Here we show that vascular endothelial cells can transform into multipotent stem-like cells by an activin-like kinase-2 (ALK2) receptor-dependent mechanism. ... More
Herpes simplex virus glycoprotein D interferes with binding of herpesvirus entry mediator to its ligands through downregulation and direct competition.
To initiate membrane fusion and virus entry, herpes simplex virus (HSV) gD binds to a cellular receptor such as herpesvirus entry mediator (HVEM). HVEM is a tumor necrosis factor (TNF) receptor family member with four natural ligands that either stimulate (LIGHT and LTa) or inhibit (BTLA and CD160) T cell ... More
Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect.
Intravenous (IV) stem cell delivery for regenerative tissue therapy has been increasingly used in both experimental and clinical trials. However, recent data suggest that the majority of administered stem cells are initially trapped in the lungs. We sought to investigate variables that may affect this pulmonary first-pass effect. In anesthetized ... More
Inclusion formation and neuronal cell death through neuron-to-neuron transmission of alpha-synuclein.
AuthorsDesplats P, Lee HJ, Bae EJ, Patrick C, Rockenstein E, Crews L, Spencer B, Masliah E, Lee SJ,
JournalProc Natl Acad Sci U S A
PubMed ID19651612
Neuronal accumulation of alpha-synuclein and Lewy body formation are characteristic to many neurodegenerative diseases, including Parkinson's disease (PD). This Lewy pathology appears to spread throughout the brain as the disease progresses. Furthermore, recent studies showed the occurrence of Lewy pathology in neurons grafted into the brains of PD patients, suggesting ... More
Intravenous mesenchymal stem cell therapy for traumatic brain injury.
Cell therapy has shown preclinical promise in the treatment of many diseases, and its application is being translated to the clinical arena. Intravenous mesenchymal stem cell (MSC) therapy has been shown to improve functional recovery after traumatic brain injury (TBI). Herein, the authors report on their attempts to reproduce such ... More
Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain.
AuthorsVasudevan A, Long JE, Crandall JE, Rubenstein JL, Bhide PG,
JournalNat Neurosci
PubMed ID18344991
Prevailing notions of cerebral vascularization imply that blood vessels sprout passively into the brain parenchyma from pial vascular plexuses to meet metabolic needs of growing neuronal populations. Endothelial cells, building blocks of blood vessels, are thought to be homogeneous in the brain with respect to their origins, gene expression patterns ... More
Quantum dot imaging for embryonic stem cells.
AuthorsLin S, Xie X, Patel MR, Yang YH, Li Z, Cao F, Gheysens O, Zhang Y, Gambhir SS, Rao JH, Wu JC
JournalBMC Biotechnol
PubMed ID17925032
ABSTRACT: BACKGROUND: Semiconductor quantum dots (QDs) hold growing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate the in vivo multiplex and long-term imaging of mouse embryonic stem (ES) cells labeled with Qtracker delivered quantum dots (QDs). RESULTS: Murine ES cells were ... More
Pulmonary artery adventitial fibroblasts cooperate with vasa vasorum endothelial cells to regulate vasa vasorum neovascularization: a process mediated by hypoxia and endothelin-1.
AuthorsDavie NJ, Gerasimovskaya EV, Hofmeister SE, Richman AP, Jones PL, Reeves JT, Stenmark KR
JournalAm J Pathol
PubMed ID16723696
The precise cellular and molecular mechanisms regulating adventitial vasa vasorum neovascularization, which occurs in the pulmonary arterial circulation in response to hypoxia, remain unknown. Here, using a technique to isolate and culture adventitial fibroblasts (AdvFBs) and vasa vasorum endothelial cells (VVECs) from the adventitia of pulmonary arteries, we report that ... More
Behavioral profiling of human transitional cell carcinoma ex vivo.
AuthorsEstrada CR, Salanga M, Bielenberg DR, Harrell WB, Zurakowski D, Zhu X, Palmer MR, Freeman MR, Adam RM
JournalCancer Res
PubMed ID16540657
Outcome studies of many types of cancer have revealed that tumors of indistinguishable histologic appearance may differ significantly in aggressiveness and in their response to therapy. A strategy that would enable early identification of patients at high risk for disease progression and allow screening of multiple therapeutic agents simultaneously for ... More
Niche-dependent translineage commitment of endothelial progenitor cells, not cell fusion in general, into myocardial lineage cells.
AuthorsMurasawa S, Kawamoto A, Horii M, Nakamori S, Asahara T
JournalArterioscler Thromb Vasc Biol
PubMed ID15860746
OBJECTIVE: Previous studies from our laboratory have shown therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial ischemia. Our purpose was to investigate the mechanisms regulating EPC contribution to myocardial regeneration. METHODS AND RESULTS: To evaluate niche-dependent expression profiles of EPCs in vitro, we performed coculture using ... More
Tracking and Imaging of Transplanted Stem Cells in Animals.
AuthorsChikate TR, Tang L
JournalMethods Mol Biol
PubMed ID31872333
'The shortage of organ donors has contributed to the rapid development of cell-based therapy in which stem cells are transplanted and administered to repair or regenerate damaged tissues or organs. The common sources of stem cells are embryonic, mesenchymal, stromal, and induced pluripotent cells. Despite the popularity of stem cell ... More
Quantum dot labeling and tracking of cultured limbal epithelial cell transplants in vitro.
Authors
JournalInvest Ophthalmol Vis Sci
PubMed ID26024089
Major Vault Protein Prevents Atherosclerotic Plaque Destabilization by Suppressing Macrophage ASK1-JNK Signaling.
Authors
JournalArterioscler Thromb Vasc Biol
PubMed ID35387478
Lipid Receptor G2A-Mediated Signal Pathway Plays a Critical Role in Inflammatory Response by Promoting Classical Macrophage Activation.
Authors
JournalJ Immunol
PubMed ID33941654
Intrarenal arterial administration of human umbilical cord-derived mesenchymal stem cells effectively preserved the residual renal function of diabetic kidney disease in rat.
Authors
JournalStem Cell Res Ther
PubMed ID35526048
Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis.
Authors
JournalStem Cells
PubMed ID17495112
Detection of Temperature Difference in Neuronal Cells.
Authors
JournalSci Rep
PubMed ID26925874
Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis.
Authors
JournalCell Rep
PubMed ID32937134
Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry."
Authors
JournalInt J Mol Sci
PubMed ID34445669
Nanoparticle vesicle encoding for imaging and tracking cell populations.
Authors
JournalNat Methods
PubMed ID25218182
Unbiased peptoid combinatorial cell screen identifies plectin protein as a potential biomarker for lung cancer stem cells.
AuthorsRaymond AC, Gao B, Girard L, Minna JD, Gomika Udugamasooriya D
JournalSci Rep
PubMed ID31628412
Tumors often contain a small subset of drug-resisting, self-renewing, and highly metastatic cells called tumor initiating cells or cancer stem cells (CSCs). To develop new approaches to detecting and targeting lung cancer CSCs, we applied an "unbiased" peptoid combinatorial cell screen to identify highly specific ligands that bind a CSC ... More
In Vivo Generation of Gut-Homing Regulatory T Cells for the Suppression of Colitis.
AuthorsXu Y, Cheng Y, Baylink DJ, Wasnik S, Goel G, Huang M, Cao H, Qin X, Lau KW, Chan C, Koch A, Pham LH, Zhang J, Li CH, Wang X, Berumen EC, Smith J, Tang X
JournalJ Immunol
PubMed ID31053627
Current therapies for gut inflammation have not reached the desired specificity and are attended by unintended immune suppression. This study aimed to provide evidence for supporting a hypothesis that direct in vivo augmentation of the induction of gut-homing regulatory T (Treg) cells is a strategy of expected specificity for the ... More
Adult human cardiac stem cell supplementation effectively increases contractile function and maturation in human engineered cardiac tissues.
AuthorsMurphy JF, Mayourian J, Stillitano F, Munawar S, Broughton KM, Agullo-Pascual E, Sussman MA, Hajjar RJ, Costa KD, Turnbull IC
JournalStem Cell Res Ther
PubMed ID31801634
Delivery of stem cells to the failing heart is a promising therapeutic strategy. However, the improvement in cardiac function in animal studies has not fully translated to humans. To help bridge the gap between species, we investigated the effects of adult human cardiac stem cells (hCSCs) on contractile function of ... More
Intravital visualization of interactions of murine Peyer's patch-resident dendritic cells with M cells.
AuthorsKolesnikov M, Curato C, Zupancic E, Florindo H, Shakhar G, Jung S
JournalEur J Immunol
PubMed ID31856298
The small intestine hosts specialized lymphoid structures, the Peyer's patches, that face the gut lumen and are overlaid with unique epithelial cells, called microfold (M) cells. M cells are considered to constitute an important route for antigen uptake in the mucosal immune system. Here, we used intravital microscopy to define ... More