When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?
Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.
Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates.
Authors:Kingeter LM, Schaefer BC,
Journal:BMC Biotechnol
PubMed ID:19463154
'BACKGROUND: Confocal microscopy is a widely employed methodology in cellular biology, commonly used for investigating biological organization at the cellular and sub-cellular level. Most basic confocal microscopes are equipped to cleanly discriminate no more than four fluorophores in a given sample, limiting the utility of this method for co-localization, co-expression, ... More
Human stem cell delivery for treatment of large segmental bone defects.
Authors:Dupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the ... More
Role of inflammation and insulin resistance in endothelial progenitor cell dysfunction.
'Endothelial progenitor cells (EPCs) are decreased in number and function in type 2 diabetes. Mechanisms by which this dysfunction occurs are largely unknown. We tested the hypothesis that a chronic inflammatory environment leads to insulin signaling defects in EPCs and thereby reduces their survival. Modifying EPCs by a knockdown of ... More
Glycoprotein D actively induces rapid internalization of two nectin-1 isoforms during herpes simplex virus entry.
Authors:Stiles KM, Krummenacher C,
Journal:Virology
PubMed ID:20089288
'Entry of herpes simplex virus (HSV) occurs either by fusion at the plasma membrane or by endocytosis and fusion with an endosome. Binding of glycoprotein D (gD) to a receptor such as nectin-1 is essential in both cases. We show that virion gD triggered the rapid down-regulation of nectin-1 with ... More
Alzheimer disease macrophages shuttle amyloid-beta from neurons to vessels, contributing to amyloid angiopathy.
Authors:Zaghi J, Goldenson B, Inayathullah M, Lossinsky AS, Masoumi A, Avagyan H, Mahanian M, Bernas M, Weinand M, Rosenthal MJ, Espinosa-Jeffrey A, de Vellis J, Teplow DB, Fiala M,
Journal:Acta Neuropathol
PubMed ID:19139910
'Neuronal accumulation of oligomeric amyloid-beta (Alphabeta) is considered the proximal cause of neuronal demise in Alzheimer disease (AD) patients. Blood-borne macrophages might reduce Abeta stress to neurons by immigration into the brain and phagocytosis of Alphabeta. We tested migration and export across a blood-brain barrier model, and phagocytosis and clearance ... More