Yeast Nitrogen Base - FAQs

在货号为Q30007的包装中，每包所含试剂可制备500 毫升用于Pichia 培养基的10X YNB，或1,000 毫升用于S. cerevisiae培养基的10X YNB（因为Pichia 培养基的用量是S. cerevisiae培养基的2倍）。以下是1X YNB的配方：

硫酸铵 5 g
生物素 0.002 mg
泛酸钙 0.4 mg
叶酸 0.002 mg
肌醇 2 mg
盐酸 0.4 mg
对氨基苯甲酸 0.2 mg
盐酸吡哆辛 0.4 mg
核黄素 0.2 mg
盐酸硫胺素 0.4 mg
硼酸 0.5 mg
硫酸铜 0.4 mg
碘化钾 0.1 mg
六水三氯化铁 0.2 mg
一水硫酸锰 0.4 mg
二水钼酸钠 0.2 mg
一水硫酸锌 0.4 mg
磷酸二氢钾 1 g
硫酸镁 0.5 g
氯化钠 0.1 g
氯化钙 0.1 mg

可以，含硫酸铵且无氨基酸的YNB培养基可通过高压蒸汽灭菌。

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg

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Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.