Analysis of in vivo expressed genes in Mannheimia haemolytica A1.
AuthorsLo RY, Sathiamoorthy S, Shewen PE
JournalFEMS Microbiol Lett
PubMed ID16984402
'The expression of Mannheimia haemolytica A1 genes during in vivo growth was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) using total RNA extracted directly from M. haemolytica A1 recovered from pneumonic lungs of cattle. Primers specific for three groups of genes were used. Group 1 includes virulence-related genes: lktC, tbpB, ... More
High transcript level of fatty acid-binding protein 11 but not of very low-density lipoprotein receptor is correlated to ovarian follicle atresia in a teleost fish (Solea senegalensis).
AuthorsAgulleiro MJ, André M, Morais S, Cerdà J, Babin PJ,
JournalBiol Reprod
PubMed ID17554079
'Transcripts encoding a fatty acid-binding protein (FABP), Fabp11, and two isoforms of very low-density lipoprotein receptor (Vldlr; vitellogenin receptor) were characterized from the ovary of Senegalese sole (Solea senegalensis). Phylogenetic analyses of vertebrate FABPs demonstrated that Senegalese sole Fabp11, as zebrafish (Danio rerio) homologous sequences, is part of a newly ... More
Function of periplasmic hydrogenases in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.
AuthorsCaffrey SM, Park HS, Voordouw JK, He Z, Zhou J, Voordouw G,
JournalJ Bacteriol
PubMed ID17601789
'The sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough possesses four periplasmic hydrogenases to facilitate the oxidation of molecular hydrogen. These include an [Fe] hydrogenase, an [NiFeSe] hydrogenase, and two [NiFe] hydrogenases encoded by the hyd, hys, hyn1, and hyn2 genes, respectively. In order to understand their cellular functions, we have compared the ... More
Utrophin up-regulation by an artificial transcription factor in transgenic mice.
AuthorsMattei E, Corbi N, Di Certo MG, Strimpakos G, Severini C, Onori A, Desantis A, Libri V, Buontempo S, Floridi A, Fanciulli M, Baban D, Davies KE, Passananti C,
JournalPLoS ONE
PubMed ID17712422
Duchenne Muscular Dystrophy (DMD) is a severe muscle degenerative disease, due to absence of dystrophin. There is currently no effective treatment for DMD. Our aim is to up-regulate the expression level of the dystrophin related gene utrophin in DMD, complementing in this way the lack of dystrophin functions. To this ... More
Preparation of genomic DNA from a single species of uncultured magnetotactic bacterium by multiple-displacement amplification.
AuthorsArakaki A, Shibusawa M, Hosokawa M, Matsunaga T,
JournalAppl Environ Microbiol
PubMed ID20081000
Magnetotactic bacteria comprise a phylogenetically diverse group that is capable of synthesizing intracellular magnetic particles. Although various morphotypes of magnetotactic bacteria have been observed in the environment, bacterial strains available in pure culture are currently limited to a few genera due to difficulties in their enrichment and cultivation. In order ... More
Comparative analysis of the soluble organic matrix of axial skeleton and sclerites of Corallium rubrum: insights for biomineralization.
AuthorsDebreuil J, Tambutté S, Zoccola D, Segonds N, Techer N, Allemand D, Tambutté E,
JournalComp Biochem Physiol B Biochem Mol Biol
PubMed ID21281736
We analysed the soluble organic matrix (SOM) of two biominerals formed by the same organism but differing by their morphological characteristics: the axial skeleton and the sclerites of Corallium rubrum. The results of 1D SDS-PAGE electrophoresis show for the two biominerals that SOM proteins bands have similar apparent molecular weight ... More
Sensitive multiplex real-time reverse transcription-PCR assay for the detection of human and animal noroviruses in clinical and environmental samples.
AuthorsWolf S, Williamson WM, Hewitt J, Rivera-Aban M, Lin S, Ball A, Scholes P, Greening GE,
JournalAppl Environ Microbiol
PubMed ID17616614
In this study, we developed a triplex real-time reverse transcription-PCR (RT-PCR)-based method that detects and distinguishes between noroviruses belonging to genogroups I, II, and III and that targets the junction between the regions of open reading frame 1 (ORF1) and ORF2. This is the first assay to include all three ... More