Q: Quant-iT RNA检测试剂可以用于定量地高辛标记的RNA吗？

可以的，该标记物不会干扰信号。

Question 1

Quant-iT RNA检测试剂可以用于定量地高辛标记的RNA吗？

Accepted Answer

可以的，该标记物不会干扰信号。

Question 2

Qubit Assay中的染料其激发/发射波长是多少？

Accepted Answer

精确的激发/发射波长信息室保密的，这里是激发/发射波长的大致信息：

- Qubit dsDNA HS Assay: ~500 nm/ ~530 nm
- Qubit dsDNA BR Assay: ~510 nm/ ~530 nm
- Qubit ssDNA Assay: ~490 nm/ ~520 nm
- Qubit RNA HS Assay: ~640 nm/ ~670 nm
- Qubit RNA BR Assay: ~640 nm/ ~670 nm
- Qubit microRNA Assay: ~500 nm/ ~520 nm
- Qubit Protein Assay: ~470 nm/ ~570 nm

Question 3

Can the Quant-iT RNA assay be used to quantitate RNA labeled with digoxigenin?

Accepted Answer

Yes, the tag does not interfere with the signal.

Question 4

What are the excitation/emission wavelengths for dyes in the Qubit Assays?

Accepted Answer

The exact excitation/emission wavelength information is proprietary. Here are the approximate excitation/emission wavelengths:

- Qubit dsDNA HS Assay: ~500 nm/ ~530 nm
- Qubit dsDNA BR Assay: ~510 nm/ ~530 nm
- Qubit ssDNA Assay: ~490 nm/ ~520 nm
- Qubit RNA HS Assay: ~640 nm/ ~670 nm
- Qubit RNA BR Assay: ~640 nm/ ~670 nm
- Qubit microRNA Assay: ~500 nm/ ~520 nm
- Qubit Protein Assay: ~470 nm/ ~570 nm

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 5

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

Accepted Answer

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 6

Can the Qubit kits give an indication of sample quality in nucleic acid samples?

Accepted Answer

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.