Quant-iT™ dsDNA 定量试剂盒,高灵敏度 (HS) 和宽范围 (BR)
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Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Quant-iT™ dsDNA 定量试剂盒,高灵敏度 (HS) 和宽范围 (BR)
Invitrogen™

Quant-iT™ dsDNA 定量试剂盒,高灵敏度 (HS) 和宽范围 (BR)

相较于 RNA,Quant-iT dsDNA 定量试剂盒(高灵敏度和宽范围)对双链 DNA 具有高度选择性,分别在 0.2-100 ng 和 2-1000 ng DNA 范围内产生线性荧光信号。
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货号检测定量范围
Q33120dsDNA 定量,高灵敏度0.2 至 100 ng
Q33130dsDNA 定量,宽范围4 至 1000 ng
货号 Q33120
价格(CNY)
5,084.00
飞享价
Ends: 31-Dec-2025
6,468.00
共减 1,384.00 (21%)
Each
添加至购物车
检测:
dsDNA 定量,高灵敏度
定量范围:
0.2 至 100 ng
价格(CNY)
5,084.00
飞享价
Ends: 31-Dec-2025
6,468.00
共减 1,384.00 (21%)
Each
添加至购物车

使用 Quant-iT dsDNA 定量试剂盒可轻松、快速地进行 dsDNA 定量。Quant-iT 高灵敏度 dsDNA 定量试剂盒和 Quant-iT 宽范围 dsDNA 定量试剂盒均提供浓缩测定试剂、稀释缓冲液和预稀释的 DNA 标准品。相较于 RNA,这些 DNA 定量试剂盒对双链 DNA 具有高度选择性,在 0.2–100 ng DNA(HS dsDNA 定量试剂盒)和 4–1000 ng DNA(BR dsDNA 定量试剂盒)范围内,荧光信号呈线性。

Quant-iT dsDNA 高灵敏度定量试剂盒和 Quant-iT dsDNA 宽范围定量试剂盒使 DNA 定量简单、准确。这些试剂盒提供了浓缩的测定试剂、稀释缓冲液以及预稀释的 DNA 标准品。只需按 1:200 的比例稀释试剂,向微孔板的孔中添加 200 µL,加入 1–20 µL 样品,混合,然后读取荧光信号即可。

相较于 RNA,这些定量试剂盒对双链 DNA 具有高度选择性,在 0.2–100 ng(HS 定量试剂盒)或 4–1000 ng(BR 定量试剂盒)范围内,荧光信号与 DNA 含量呈线性关系。测定均在室温下进行,信号可保持稳定三小时。这些定量试剂盒对于常见污染物(如盐、溶剂、去污剂和蛋白)具有良好的耐受性。

仅供科研使用。不可用于诊断程序。
规格
检测dsDNA 定量,高灵敏度
适用于(设备)微孔板读数仪
反应次数1000次测定(200 μL 测定体积)
产品线Quant-iT
定量范围0.2 至 100 ng
数量1 kit
运输条件室温
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

为什么我用Qubit Assay检测时会得到负的荧光值?

负的荧光值物理上是不可能的。它是由于软件自动扣除背景信号而造成的假象。这意味着你的荧光计检测到背景信号并将其扣除从而牺牲了真实数据。务必做一个仅有缓冲液的对照并评估信号的类型。你可能需要换用另外一块板。

我有一个Quant-iT DNA试剂盒,而想在Qubit荧光计上使用。可以吗?

是的,使用手册中有关于此项应用的说明。你将使用0 ng/μL lambda dsDNA HS标准品制备Standard #1。你将稀释一个10 ng/μL lambda dsDNA HS标准品得到Standard #2。然后你准备样本并将它们和上述的两点标准曲线进行比较。Quant-iT dsDNA BR试剂盒也可以用类似的方式使用。

这些Quant-iT试剂盒的适用pH范围是多少?

试剂盒中的缓冲液将保证合适的pH值范围,即使您的DNA处于这一pH范围之外也没关系,因为检测中所用的缓冲液体积至少超过样本体积的10倍。

我试图对一些带有荧光基团标记的DNA进行定量。这可行吗?

PicoGreen染料和其它基于荧光定量的试剂不建议用于对染料偶联的核酸进行定量。核酸上携带的染料基团会干扰定量试剂的结合或荧光产生。

DNA长度对dsDNA检测结果有影响吗?

大致在20-mer或更短范围内的链信号水平较低。对于大部分由短链组成的dsDNA样本,仍可使用试剂,但应使用与样本长度相当的dsDNA标准品。

引用和文献 (32)

引用和文献
Abstract
Natural transformation of Myxococcus xanthus.
Authors:Wang J, Hu W, Lux R, He X, Li Y, Shi W,
Journal:J Bacteriol
PubMed ID:21378184
'Myxococcus xanthus belongs to the delta class of the proteobacteria and is notable for its complex life-style with social behaviors and relatively large genome. Although previous observations have suggested the existence of horizontal gene transfer in M. xanthus, its ability to take up exogenous DNA via natural transformation has not ... More
Gastroenteritis outbreak caused by waterborne norovirus at a New Zealand ski resort.
Authors:Hewitt J, Bell D, Simmons GC, Rivera-Aban M, Wolf S, Greening GE,
Journal:Appl Environ Microbiol
PubMed ID:17965205
'In July 2006, public health services investigated an outbreak of acute gastroenteritis among staff and visitors of a popular ski resort in southern New Zealand. The source of the outbreak was a drinking water supply contaminated by human sewage. The virological component of the investigation played a major role in ... More
Expression/localization patterns of sirtuins (SIRT1, SIRT2, and SIRT7) during progression of cervical cancer and effects of sirtuin inhibitors on growth of cervical cancer cells.
Authors:Singh S, Kumar PU, Thakur S, Kiran S, Sen B, Sharma S, Rao VV, Poongothai AR, Ramakrishna G
Journal:
PubMed ID:25794641
'Sirtuins belong to the family of class III histone deacetylases; its role in neoplasia is controversial as both tumor-suppressive and promoting functions have been reported. There are very few reports available, where expressions of sirtuin isoforms are comprehensively analyzed during neoplasia. Therefore, in the present study, the expression of SIRT1, ... More
The altered landscape of the human skin microbiome in patients with primary immunodeficiencies.
Authors:Oh J, Freeman AF, Park M, Sokolic R, Candotti F, Holland SM, Segre JA, Kong HH,
Journal:
PubMed ID:24170601
'While landmark studies have shown that microbiota activate and educate host immunity, how immune systems shape microbiomes and contribute to disease is incompletely characterized. Primary immunodeficiency (PID) patients suffer recurrent microbial infections, providing a unique opportunity to address this issue. To investigate the potential influence of host immunity on the ... More
Effect of the metabolic environment at key stages of follicle development in cattle: focus on steroid biosynthesis.
Authors:Walsh SW, Mehta JP, McGettigan PA, Browne JA, Forde N, Alibrahim RM, Mulligan FJ, Loftus B, Crowe MA, Matthews D, Diskin M, Mihm M, Evans AC,
Journal:Physiol Genomics
PubMed ID:22414914
'Cellular mechanisms that contribute to low estradiol concentrations produced by the preovulatory ovarian follicle in cattle with a compromised metabolic status are largely unknown. To gain insight into the main metabolic mechanisms affecting preovulatory follicle function, two different animal models were used. Experiment 1 compared Holstein-Friesian nonlactating heifers (n = ... More