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引用和文献 (16)
Invitrogen™
Quant-iT™ dsDNA 定量试剂盒，高灵敏度 (HS) 和宽范围 (BR)
相较于 RNA，Quant-iT dsDNA 定量试剂盒（高灵敏度和宽范围）对双链 DNA 具有高度选择性，分别在 0.2-100 ng 和 2-1000 ng DNA 范围内产生线性荧光信号。
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| 货号 | 检测 | 定量范围 |
|---|---|---|
| Q33130 | dsDNA 定量，宽范围 | 4 至 1000 ng |
| Q33120 | dsDNA 定量，高灵敏度 | 0.2 至 100 ng |
货号 Q33130
价格（CNY)
5,095.93
飞享价
Ends: 31-Dec-2026
6,483.00共减 1,387.07 (21%)
Each
检测:
dsDNA 定量，宽范围
定量范围:
4 至 1000 ng
价格（CNY)
5,095.93
飞享价
Ends: 31-Dec-2026
6,483.00共减 1,387.07 (21%)
Each
使用 Quant-iT dsDNA 定量试剂盒可轻松、快速地进行 dsDNA 定量。Quant-iT 高灵敏度 dsDNA 定量试剂盒和 Quant-iT 宽范围 dsDNA 定量试剂盒均提供浓缩测定试剂、稀释缓冲液和预稀释的 DNA 标准品。相较于 RNA，这些 DNA 定量试剂盒对双链 DNA 具有高度选择性，在 0.2–100 ng DNA（HS dsDNA 定量试剂盒）和 4–1000 ng DNA（BR dsDNA 定量试剂盒）范围内，荧光信号呈线性。
Quant-iT dsDNA 高灵敏度定量试剂盒和 Quant-iT dsDNA 宽范围定量试剂盒使 DNA 定量简单、准确。这些试剂盒提供了浓缩的测定试剂、稀释缓冲液以及预稀释的 DNA 标准品。只需按 1:200 的比例稀释试剂，向微孔板的孔中添加 200 µL，加入 1–20 µL 样品，混合，然后读取荧光信号即可。
相较于 RNA，这些定量试剂盒对双链 DNA 具有高度选择性，在 0.2–100 ng（HS 定量试剂盒）或 4–1000 ng（BR 定量试剂盒）范围内，荧光信号与 DNA 含量呈线性关系。测定均在室温下进行，信号可保持稳定三小时。这些定量试剂盒对于常见污染物（如盐、溶剂、去污剂和蛋白）具有良好的耐受性。
相较于 RNA，这些定量试剂盒对双链 DNA 具有高度选择性，在 0.2–100 ng（HS 定量试剂盒）或 4–1000 ng（BR 定量试剂盒）范围内，荧光信号与 DNA 含量呈线性关系。测定均在室温下进行，信号可保持稳定三小时。这些定量试剂盒对于常见污染物（如盐、溶剂、去污剂和蛋白）具有良好的耐受性。
仅供科研使用。不可用于诊断程序。
规格
检测dsDNA 定量，宽范围
适用于（设备）微孔板读数仪
反应次数1000次测定（200 μL 测定体积）
产品线Quant-iT
定量范围4 至 1000 ng
数量1 kit
运输条件室温
检测方法荧光
Unit SizeEach
常见问题解答 (FAQ)
为什么我用Qubit Assay检测时会得到负的荧光值？
我有一个Quant-iT DNA试剂盒，而想在Qubit荧光计上使用。可以吗？
这些Quant-iT试剂盒的适用pH范围是多少？
我试图对一些带有荧光基团标记的DNA进行定量。这可行吗？
DNA长度对dsDNA检测结果有影响吗？
引用和文献 (16)
引用和文献
Abstract
3D chromatin conformation correlates with replication timing and is conserved in resting cells.
Journal:Nucleic Acids Res
PubMed ID:22879376
'Although chromatin folding is known to be of functional importance to control the gene expression program, less is known regarding its interplay with DNA replication. Here, using Circular Chromatin Conformation Capture combined with high-throughput sequencing, we identified megabase-sized self-interacting domains in the nucleus of a human lymphoblastoid cell line, as
A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr/gr deletion.
Journal:Hum Mol Genet
PubMed ID:23307928
'AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was determined to be a potential genetic modifier of spermatogenesis. We performed a large-scale cohort study to investigate the effect of
Gene expression profiling in insulinomas of Men1 beta-cell mutant mice reveals early genetic and epigenetic events involved in pancreatic beta-cell tumorigenesis.
Journal:Endocr Relat Cancer
PubMed ID:17158767
'Mutations of the MEN1 gene lead to the occurrence of multiple endocrine neoplasia type 1 (MEN1). To gain insights into the mechanisms of the tumorigenesis related to MEN1 inactivation, we have used mice in which the Men1 gene was specifically disrupted in pancreatic beta-cells. In these mice, we observed full
Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations.
Journal:Appl Environ Microbiol
PubMed ID:22492451
'Five essential oils (EOs), namely, clove oil (CLO), eucalyptus oil (EUO), garlic oil (GAO), origanum oil (ORO), and peppermint oil (PEO), were tested in vitro at 3 different doses (0.25, 0.50, and 1.0 g/liter) for their effect on methane production, fermentation, and select groups of ruminal microbes, including total bacteria,
Normal variants of Microcephalin and ASPM do not account for brain size variability.
Journal:Hum Mol Genet
PubMed ID:16687438
'Normal human brain volume is heritable. The genes responsible for variation in brain volume are not known. Microcephalin (MCPH1) and ASPM (abnormal spindle-like microcephaly associated) have been proposed as candidate genes as mutations in both genes are associated with microcephaly, and common variants of each gene are apparently under strong