Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒
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Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒
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Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒

用于基于荧光的蛋白定量测定检测的 Qubit 蛋白和蛋白 BR 检测试剂盒可实现对蛋白的灵敏检测和低蛋白间差异。
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货号检测定量范围足够用于
Q33211Qubit 蛋白检测试剂盒12.5 µg/mL 至 5 mg/mL100 次反应
Q33212Qubit 蛋白检测试剂盒12.5 µg/mL 至 5 mg/mL500 次反应
A50668Qubit 蛋白宽范围检测试剂盒0.1 至 20 mg/mL100 次反应
A50669Qubit 蛋白宽范围检测试剂盒0.1 至 20 mg/mL500 次反应
货号 Q33211
价格(CNY)
1,561.00
Each
添加至购物车
检测:
Qubit 蛋白检测试剂盒
定量范围:
12.5 µg/mL 至 5 mg/mL
足够用于:
100 次反应
价格(CNY)
1,561.00
Each
添加至购物车

使用两种独特的 Qubit 蛋白定量试剂盒快速准确地定量蛋白:Qubit 蛋白检测(定量 12.5 μg/mL 至 5 mg/mL 蛋白)和 Qubit 蛋白 BR 检测试剂盒(定量 0.1 至 20 mg/mL 蛋白)。这两种检测均可兼容多种常见污染物(还原试剂、盐和核酸)。蛋白 BR 检测试剂盒也可耐受去污剂。

Qubit 蛋白检测试剂盒
Qubit 蛋白检测试剂盒专门设计用于配合任何 Qubit 荧光计使用。使用 1-20 µL 样品时,该检测可定量测定 12.5 µg/mL 至 5 mg/mL 范围内的样品,并且蛋白间差异较低。

Qubit 蛋白检测试剂盒对蛋白具有高度选择性,并且在存在还原试剂但不存在大量去污剂的情况下具有高准确度。该检测对于常见污染物(如还原试剂(例如 DTT、β-巯基乙醇)、盐、游离核苷酸、氨基酸、溶剂或 DNA 等,但不包括去污剂)具有很好的适应性。用于其他污染物时需要轻微修改方案。

该试剂盒提供浓缩的检测试剂、稀释缓冲液和预稀释的 BSA 标准品。只需使用提供的缓冲液对试剂进行稀释,加入您的样品(1 µL 至 20 µL 的任意体积均可接受),然后即可读取浓度。

应选择哪种产品进行荧光蛋白定量测定?
•1–20 份样品:将该 Qubit 蛋白检测试剂盒与 Qubit 荧光计配合使用
• 20–2000 份样品:将 Quant-iT 蛋白检测试剂盒与微孔板读数仪配合使用

Qubit 蛋白 BR 检测试剂盒
Qubit 蛋白 BR 检测试剂盒提供了一种快速、简便、可靠的方法,使用 Qubit 4 荧光计来测定纯化蛋白、裂解物、血清、血浆或复杂蛋白混合物的蛋白浓度。该蛋白试剂盒可在较宽范围 (0.1–20 mg/mL) 内进行准确的蛋白定量测定,使大多数样品能够以纯品(未稀释)形式使用,免除了传统蛋白定量测定方法所需的推测工作和稀释步骤。该检测可兼容多种常见污染物,如去污剂、还原试剂(例如 DTT、β-巯基乙醇)、盐和核酸。

Qubit 蛋白 BR 检测试剂盒的主要特点包括:
宽动态范围—0.1-20 mg/mL,测定样品时无需稀释
两点标准曲线—免除耗时的标准品制备
自动浓度测定—即时接收蛋白浓度;无需离线计算
兼容—在存在还原试剂、去污剂和其他常见缓冲液组分时可保持准确度

Qubit 蛋白 BR 检测试剂盒配有两种校准标准品、蛋白 BR 检测缓冲液和蛋白 BR 检测试剂。只需将 150–160 µL 蛋白 BR 检测缓冲液添加至 10 或 20 µL 样品中,然后添加 30 µL 蛋白 BR 检测试剂。在室温下孵育10分钟。然后,将样品插入 Qubit 4 仪器并读取浓度。


• Qubit 蛋白检测试剂盒可与 Qubit 1.0、Qubit 2.0、Qubit 3、Qubit 4 以及 Qubit Flex 荧光计配合使用
• Qubit 蛋白 BR 检测试剂盒是一种基于荧光的检测试剂盒,仅可与 Qubit 4 荧光计配合使用
• 需要 500 µL 薄壁透明 PCR 管(货号Q32856),但其未包含在蛋白检测和 BR 检测试剂盒中

仅供科研使用。不可用于诊断程序。
规格
检测Qubit 蛋白检测试剂盒
适用于(设备)Qubit 荧光计、微孔板读数仪
产品线Qubit™
产品类型蛋白定量检测试剂盒
定量范围12.5 µg/mL 至 5 mg/mL
数量100 次检测
运输条件室温
足够用于100 次反应
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Can the Qubit kits give an indication of sample quality in nucleic acid samples?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.

Can I use the Quant-iT and Qubit Kits with other fluorometers?

All Quant-iT and Qubit kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won't have access to the algorithm in the Qubit fluorometer for generating the standard curve provided by the instrument, instead, you must make a few dilutions of the highest standard DNA or RNA (Standard #2) in the Qubit kits to generate a standard curve with multiple data points.

Can I use the original Quant-iT Kits with the Qubit Fluorometer?

No, we do not recommend this. Some of the dyes in the original Quant-iT kits (those NOT listed as “for use with the Qubit fluorometer”) are not compatible with the Qubit Fluorometer. In addition, the new Quant-iT kits (labeled as “for use with the Qubit Fluorometer”) have standards formulated to be compatible with the Qubit fluorometer internal algorithms for the respective assays. The Qubit Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.

Is 1X RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1mM EDTA, pH 7.4) compatible with the Qubit Protein Assay (Cat. No. Q33211/Q33212)?

The concentration of detergents in 1x RIPA buffer is quite high, therefore, we do not recommend that you use this kit with samples in RIPA. The Qubit Protein Assay has a tolerance for detergents present only in very low amounts. Please see the user guide for the tolerance of this assay for detergents here. The Qubit Protein Broad Range (BR) Assay Kit (A50668) has a much higher tolerance for detergents, e.g. RIPA buffer can be used undiluted. Please see this link.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.