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查看更多产品信息 Qubit™ 4 NGS Starter Kit - FAQs (Q33228)
40 个常见问题解答
分光光度计不能区分完整核酸、小片段、降解样本，以及单核苷酸。试剂盒中的染料仅检测长度超过20mer左右的完整核酸链。试剂盒仅识别完整DNA或RNA，而忽略降解样本。
请从我们的网站下载并安装最新版本的Qubit固件（https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources.html）
对Qubit 1.0, 2.0 和 3.0荧光计，去这个链接下载：https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources/previous-qubit-models.html
对于Qubit 1.0仪器，如果它和电脑相联，那么将它和电脑的连接解除，拔掉电源，等待至少10秒，然后重新插上电源。
对于Qubit 2.0，3.0，4.0 仪器，如果可能，关闭电源或拔掉电源，等待至少10秒，然后重新插上电源。在固件升级过程中，请不要将仪器电源拔掉或将USB闪存拔掉。
如果可能，你可以试试使用较大体积的样本（最多20µL）。此外，如果你使用的是Qubit DNA BR检测试剂盒或Qubit RNA BR检测试剂盒，可以试试Qubit DNA HS检测试剂盒或Qubit RNA HS检测试剂盒，因为它们的灵敏度更高。
稀释你的样本，或者使用较小体积的样本，重新检测。此外，如果你使用的是Qubit DNA HS检测试剂盒或Qubit RNA HS检测试剂盒，可以试试Qubit DNA BR检测试剂盒或Qubit RNA BR检测试剂盒，因为它们的灵敏度更低。
如果出现这种情况，在“查看标准品”或"查看校准"下查看标准品和样本的原始荧光值。确认样本的荧光值落在标准品的荧光值之间（或稍稍高于最高的标准品）。如果不是，那么样本已经超过了检测的精确范围。Qubit荧光计的屏幕会显示“超出检测范围”。
这条信息出现可能有几个原因。请在您的仪器上检查如下事项：
1.在 “查看标准品”或"查看校准"下查看标准品的原始荧光值。Standard 2的荧光值是否至少是Standard 1荧光值的10到50倍。对于蛋白检测，Standard 3的荧光值是否是Standard 2荧光值的大约2倍。
2.检查你的标准品放置顺序是否正确。
3.检查你是在合适的检测管中使用了10µL的标准品和190µL的Qubit染料工作溶液。
4.对于RNA检测，可以试试新开一管Standard 2，以防已经打开的RNA标准品已经降解。
5.检查Qubit检测试剂盒以及标准品的收货日期。试剂盒从收到的日期起可以稳定使用至少6个月。如果该试剂盒已经超过一年并且Qubit荧光计不能读取标准品，那么应当换用一个新的试剂盒。
6.检查仪器是否正常工作。打开盖子。运行任一Assay的新的校准。你可以看到一个红色灯（对于RNA Assay）或一个蓝色灯（对于所有其它Assay）闪烁5次。
可以，使用手册中有关于此应用的说明。
是的。我们观察到20–30%的差别。对于不同形态的质粒DNA，我们建议使用和样本中的质粒DNA形态非常近似的标准品。欲获知创建自定义优化检测法的信息，请访问我们的网站(https://www.thermofisher.com/search/results?query=MyQubit)。
是的。对于典型的高浓度的小抽DNA（超过50 ng/μL），可以使用Qubit DNA BR检测法。对于微量质粒或少量质粒，可以使用Qubit DNA HS检测法。但是，关于构象问题请参见下一问题。
Qubit 2.0荧光计原装的USB为2GB，Qubit 3.0和4.0荧光计原装的USB为4GB。
我们将为您更换该设备。请联系我们的技术支持部门以了解详情，邮件地址LifeScience-CNTS@thermofisher.com。
不能。如果设备被拆解或者你试图修理设备则设备的保修将失效。最好电话或邮件通知我们的技术支持团队以获取帮助。
Qubit 荧光计内有两个光源——均为LED灯。它们预期的使用寿命至少是5年。
如果使用和样本一样的工作溶液，那么稀释后的标准品最多可以使用3小时。在缓冲液中稀释的Qubit试剂稳定长达3小时。如果存在，蒸发会导致染料和样品浓度更高，这将影响结果。
Invitrogen Qubit 3.0和4.0荧光计可以在一个.csv文件中存储最多相当于1000个样本的数据。
Qubit 2.0荧光计可以在一个.csv文件中存储最多200行数据。
最早的Qubit (1.0)荧光计可以在一个.csv文件中存储最多20行数据。
是的。这些试剂盒适用于所有Qubit荧光计。
是的，Qubit 4.0荧光计带有一个USB闪存盘和USB数据线，用于和电脑相连。
是的。
Qubit荧光计能够非常准确、灵敏并简便的对DNA, RNA和蛋白质进行定量。它是为分子生物学实验室设计的，用于处理稀有或难以处理的珍贵样品，以及需要精确测量的应用，如实时PCR。查看关于Qubit荧光计的更多信息(http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-fluorometer.html.html)
Absorbance readers cannot distinguish small fragments, degraded samples, or single nucleotides from intact nucleic acid. The dyes in the kits only detect intact strands longer than about a 20-mer. The kits are only recognizing intact DNA or RNA, and ignoring degraded sample.
Please download and install the most current version of the Qubit Firmware from our website: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources.html
For Qubit Fluorometers 1.0, 2.0 and 3.0 go to this web link: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources/previous-qubit-models.html
For the Qubit 1.0 instrument, if it is connected to a computer, disconnect it from the computer, unplug it, wait at least 10 seconds, then plug it back in.
For the Qubit 2.0, 3.0 and 4.0 instruments, power off or unplug the instrument if possible, wait at least 10 seconds, then plug it back in. Do not unplug the instrument or remove the USB stick during a firmware update.
You can try to use a larger amount of sample if possible (up to 20 µL). Alternatively, if you are using the Qubit DNA BR Assay or Qubit RNA BR Assay, try using the Qubit DNA HS Assay or Qubit RNA HS Assay, as these have higher sensitivity.
Dilute your sample, or use a smaller volume of sample in the assay and try again. Alternatively, if you are using the Qubit DNA HS Assay or the Qubit RNA HS Assay, try using the Qubit DNA BR Assay or Qubit RNA BR Assay, as these have lower sensitivity.
If this occurs, view the raw fluorescence values for the standards and samples under “Check Standards” or “Check Calibration”. Confirm that the values for the samples fall between the values of the standards (or a little above the highest standard). If they do not, the sample is out of the accurate range of the assay. The Qubit Fluorometer screen should indicate OUT OF RANGE.
There are several reasons why this message may appear. Please check the following on your instrument:
1.View the raw fluorescence value for the standards under “Check Standards” or “Check Calibration”. Make sure that the value of Standard 2 is at least 10- to 50-fold higher than the value of Standard 1. For the protein assay, make sure that the value of Standard 3 is about twice the value of Standard 2.
2.Check to see that you have used the tubes in the correct order.
3.Check to see that you used 10 µL of the standard and 190 µL of the Qubit dye working solution in the appropriate tubes.
4.For RNA assays, try an unopened tube of Standard 2 in case the open RNA standard tube has degraded.
5.Check the age of the Qubit assay kit and standards. Kits are stable for at least 6 months from the date of receipt. If the kit is older than a year and the Qubit Fluorometer will not read the standards, it may need to be replaced with a new kit.
6.Check to see if the instrument is working properly. Lift the lid. Run a new calibration for any assay. You should see a red light (for RNA assays) or a blue light (for all other assays) flash 5 times.
Yes, the manual has directions for this application.
Yes. We have seen a 20-30% difference. For the different forms of plasmid DNA, we recommend using a standard that more closely represents the composition of the plasmid DNA in the sample. For information on creating custom optimized assays, please visit our website (http://www.thermofisher.com/search/global/searchAction.action?query=MyQubit&resultPage=1&resultsPerPage=15&autocomplete).
Yes. Use the Qubit DNA BR Assay for a typical plasmid miniprep with a high concentration of DNA (over 50 ng/µL). Use the Qubit DNA HS Assay for plasmid rescue or methods that yield only small amounts of DNA. However, see the next question for conformation issues.
The USB that comes with the Qubit 2.0 Fluorometer is 2 GB, and the one that comes with the Qubit 3.0 and 4.0 Fluorometer is 4 GB.
We will replace the instrument. Please contact our Technical Support department for details by emailing techsupport@thermofisher.com.
No. The warranty will be voided if the instrument is disassembled or if you attempt to repair the instrument. It is best to call or email our technical support team for assistance.
There are two light sources in the Invitrogen Qubit Fluorometers - both are LEDs. They are expected to last at least 5 years.
The diluted standards can be used for up to 3 hours if using the same working solution for the samples. The Qubit reagents diluted in buffer are stable for up to 3 hours. Evaporation would result in a higher concentration of dye and sample, if present, and this would affect the results.
The Invitrogen Qubit 3.0 and 4.0 Fluorometers can store up to 1,000 samples' worth of data in a .csv file.
The Invitrogen Qubit 2.0 Fluorometer can store up to 200 lines of data in a .csv file.
The original Invitrogen Qubit (1.0) Fluorometer can store up to 20 lines of data in a .csv file.
Yes, these kits will work with all Qubit Fluorometers.
Yes, the Qubit 4.0 Fluorometer comes with both a USB and cable to connect to a computer.
Yes, the Invitrogen Qubit&trade 3.0 Fluorometer will be discontinued.
The Qubit Fluorometer quantifies DNA, RNA, and protein with superior accuracy, sensitivity, and simplicity. It is designed for molecular biology labs working with precious samples that are rare or difficult to process, and applications requiring precise measurement such as real-time PCR. See more about the Qubit Fluorometer (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-fluorometer.html.html).