Qubit™ 4 Fluorometer, with WiFi - FAQs

3 years is the standard warranty period.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

The Qubit 4 Fluorometer needs to be updated. The Qubit 4 Fluorometer can be updated with the USB that was provided with the Qubit. If you do not have the USB, we recommend using an empty 2.0GB that is FAT32 formatted. If the USB is not recognized, leave USB plugged in for a few minutes before running the update. This should give you the assay you need to run the Qubit Assay.

You can find the updates on the Technical Resources for Qubit Fluorometers page.

To access the Qubit ssDNA Assay Kit on the Qubit 4, please try selecting the Oligo option from the home screen, and the ssDNA Assay should appear there. If not, please check if you need to update your instrument firmware to the latest version v2.19, which is available for download from the Technical Resources for Qubit Fluorometers page on thermofisher.com.
For standard/preloaded Qubit assays we do not provide individual .qbt files, but they are included in the firmware itself.

To be able to use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer (Cat. No Q33238) you would need to install the latest firmware on the fluorometer. Please find the latest Qubit 4 firmware (v2.19) on the following webpage: https://downloads.thermofisher.com/Qubit4/v2.19/Qubit4_update_v2.19.pak Save the firmware update on a USB stick and upload it to the Qubit 4 Fluorometer. Once the firmware update has been installed, you will be able to see the Protein BR assay on the Qubit 4 Fluorometer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

You have to create a user profile and log in. Please follow the instructions for login below:

• Turn on the device
• From the home screen, touch the person icon on the top left corner
• Touch the “Get Started” button
• Touch the “Create Profile” button
• information for username and pin
• Log in by touching the person icon and entering the user info in the sign in fields

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Due to cybersecurity regulations, it is mandatory to create a profile on the device before all features become available. Please follow these steps:

• Click on the user icon in the upper left corner.
• Click on Get Started. The device will guide you through the steps to create a profile.
• Select a pin and confirm the creation of the profile.
• Log in with this new profile and you should now have all the features available.
You can also find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the release notes.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center

Custom assays can be designed on Qubit 3 and Qubit 4 Fluorometers. For more information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center

Due to the security settings of the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238), you will need to be logged in as a user to access the full settings menu. When not logged in as a user, options such as instrument settings, software update and system verification remain inactive.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center

You can find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the Firmware release notes.

The Qubit 4 Fluorometer, with WIFI (Cat. No. Q33238), does not require any specific routine maintenance. However, based on customer experience, we recommend the following:

• From time to time, it may be useful to check the opening of the Qubit for dust particles and dirt. If needed, you can use a lint-free cloth or lenses cleaning paper towels to wipe the inside of the Qubit with some 75%-95% ethanol.


• If you experience inaccurate measurements, regardless of the kit you use, we recommend testing the light source of the instrument with the Qubit 4 System Verification Assay Kit (Cat. No. Q33238).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

To access the necessary file for the Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits, update the Qubit 4 firmware using this link. The newest firmware contains the required assay file.

Our Qubit 4 and Qubit Flex instruments will now require users to sign in to access certain menu options (i.e., instrument settings, software update, and system verification). This is necessary to adhere to cybersecurity legal standards that were put in place in 2020. Only when signed in, are all functions of the device are accessible.

Instructions for login:
- Turn on the device
- From the home screen, touch the person icon on the top left corner
- Touch the "Get Started" button
- Touch the "Create Profile" button
- Enter information for username and pin
- Log in by touching the person icon and entering the user info in the sign in fields

To resolve this issue, create a user profile prior to measuring samples. If the problem persists, please contact technical support for further assistance at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

To connect the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238) to the Thermo Fisher Cloud, you need to have an active Thermo Fisher Connect account. You can create an account here (https://www.thermofisher.com/us/en/home/digital-science/thermo-fisher-connect.html) and then try again.

If you have an active Thermo Fisher Connect account and your instrument is connected to the internet, you can try these approaches:
1. Confirm that the date, time, and time zone are correct.
2. Try a workaround in which you sign into the local account and then sign into Thermo Fisher Connect within the export data screen.
The steps are:
    a. Go to a data set, click the checkbox next to it
    b. Export
    c. CSV or PDF
    d. Select "Cloud" and follow the on-screen instructions.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Due to the security settings of the Qubit 4 Fluorometer, with WiFi (No. Q33238), you will need to be logged in as a user to access the full settings menu.

You can find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the Qubit 4 Fluorometer Firmware v2.18 Release Notes.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

We do not have an IQ/OQ qualification service for the Qubit Fluorometer.