Can the Qubit 1X dsDNA, broad range (BR) Assay Kit (Cat. No. Q33265) be used on the Qubit 3 Fluorometer?
No, this assay is only compatible with Qubit 4 Fluorometer (Cat. No. Q33226) and Qubit Flex Fluorometer (Cat. No. Q33327).
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
What is the shelf life for the 1X dsDNA BR working solution(Cat. No. Q33262), that is part of the Qubit 1X dsDNA BR Assay (Cat Nos. Q33265, Q33266)?
The storage conditions for the 1X dsDNA BR working solution are 18–28 degrees C (room temperature). When stored as directed, the kit contents are stable for at least 6 months from delivery date. The standards should still be kept in the fridge.
After mixing the sample or the standard with the working solution, the fluorescence signal is stable for 3 hours at room temperature when samples are protected from light, following the incubation period.
Storage information can be found on page 1, and incubation time is on page 2, of the Qubit 1X dsDNA BR Assay User Guide
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
I would like to use my Qubit 4 Fluorometer with the Qubit 1X dsDNA BR Assay, but the assay is not listed as an option on the fluorometer. How can I resolve this?
The Qubit 4 Fluorometer needs to be updated. The Qubit 4 Fluorometer can be updated with the USB that was provided with the Qubit. If you do not have the USB, we recommend using an empty 2.0GB that is FAT32 formatted. If the USB is not recognized, leave USB plugged in for a few minutes before running the update. This should give you the assay you need to run the Qubit Assay.
You can find the updates on the Technical Resources for Qubit Fluorometers page.
Does the Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266)?
No. The Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) does not include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266). It must be purchased separately.
The description of the Qubit Flex Starter Packages (Cat. Nos. Q32894, Q45893, Q45894) can be found on the Qubit Flex Starter Packages product page.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
What is the expiration date for Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266)?
The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.
For more information on product storage, please refer to the user guides:
Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.