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Invitrogen™
QuantiGene™ Plex 384-well Assay Kit
The QuantiGene Plex 384-well Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 384-well plates and must be used with a target-specific QuantiGene Plex panel (sold separately).
| Catalog Number | Quantity |
|---|---|
| QP1016 | 2 Plates |
Catalog number QP1016
Price (CNY)
60,385.00
Each
Quantity:
2 Plates
Price (CNY)
60,385.00
Each
The QuantiGene Plex 384-well Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 384-well plates and must be used with a target-specific QuantiGene Plex panel (sold separately).
The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument.
Features of the QuantiGene Plex Assay include:
- Difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
- True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity
- Standardized platform—uses 96 or 384-well plate formats compatible with the INTELLIFLEX, Luminex 200, FlexMap3D, and MagPix systems
- Simple workflow—employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling
- Large inventory of validated genes—select from over 15,000 genes to create pathway and disease-themed panels
- Rapid turnaround for custom panels—if we don't have your gene(s), we can create a custom panel within two weeks
Technology
QuantiGene assays utilize branched DNA (bDNA) technology that has been used for decades in the VERSANT™ diagnostic assays for HIV-1, HCV, and HBV. First, cells are lysed or tissue samples are homogenized to release the target RNA. Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight. During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA. Third, signal amplification is performed via sequential hybridization of the bDNA preamplifier, amplifier, and label-probe molecules to the target. Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample.
Applications
QuantiGene Plex Assay applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical samples, miRNA profiling, microarray validation, predictive toxicology, and detection of translocations and fusion genes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)QuantiGene Plex Panel
For Use With (Equipment)Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product LineQuantiGene Plex
Sample TypePurified RNA, cell/blood/FFPE lysates, tissue homogenates
Shipping ConditionBlue Ice
Detection MethodFluorescence
Format384-well
Product TypeQuantiGene Plex 384-well Assay Kit
Quantity2 Plates
SpeciesUniversal
Unit SizeEach
Contents & Storage
• 200 μL Proteinase K, -20°C
• 2.1 mL Blocking Reagent, 2°C to 8°C
• 31 mL Label Probe Solution, 2°C to 8°C
• 31 mL Pre-Amplifier Solution, 2°C to 8°C
• 31 mL Amplifier Solution, 2°C to 8°C
• 31 mL SAPE Solution, 2°C to 8°C
• 24 mL Lysis Mixture, 15–30°C
• 6 mL Wash Buffer Component 1, 15–30°C
• 100 mL Wash Buffer Component 2, 15–30°C
• 120 mL10x SAPE Wash Buffer Concentrate, 15–30°C
• 2 Hybridization plates (Day 1) 384-well, 15–30°C
• 4 Manual Pressure seals (Day 1), 15–30°C
• 4 Heat seals (Day 1), 15–30°C
• 2 Magnetic Separation plates (Day 2), 15–30°C
• 10 Plates seals (Day 2), 15–30°C
Note: Prepared 1x Wash Buffers can be stored for 1 week protected from light at 4°C.