QuantiGene™ 样品处理试剂盒,培养的细胞
QuantiGene™ 样品处理试剂盒,培养的细胞
Invitrogen™

QuantiGene™ 样品处理试剂盒,培养的细胞

用于培养细胞的此 QuantiGene 样品处理试剂盒包含用于制备细胞裂解物的试剂和说明,它们用于 RNA 靶标的 QuantiGene Singleplex 和 QuantiGene Plex了解更多信息
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货号数量
QS010350 块板
QS01002块板
QS010110 Plates
QS01025 x 75 mL
货号 QS0103
价格(CNY)
14,895.00
Each
添加至购物车
数量:
50 块板
价格(CNY)
14,895.00
Each
添加至购物车
用于培养细胞的此 QuantiGene 样品处理试剂盒包含用于制备细胞裂解物的试剂和说明,它们用于 RNA 靶标的 QuantiGene Singleplex 和 QuantiGene Plex 测定试剂盒以及 DNA 靶标的 QuantiGene Plex DNA 测定试剂盒。

•易于遵循的说明
• 保存 RNA/DNA 的完整性,不会降解
• 选择与您的培养细胞样品匹配的方法

您能获得什么
用于培养细胞的 QuantiGene 样品处理试剂盒包含我们专有的裂解混合物、蛋白酶 K 和从培养细胞制备细胞裂解物的详细说明。

细胞裂解成功
影响细胞完全裂解的关键因素包括:
•裂解混合物
• 蛋白酶 K
• 涡旋或移取样品
• 裂解液在 -80°C 下储存
• 在 50°C 下孵育

用于培养细胞的 QuantiGene 样品处理试剂盒提供的方案使用这些因子的组合,以确保样品完全裂解。此外,裂解混合物为 QuantiGene 测定中的过夜杂交提供了适当的离子条件。

仅供科研使用。不可用于诊断程序。
规格
产品线QuantiGene™
产品类型样品处理试剂盒
数量50 块板
运输条件蓝冰
产品规格试剂盒
样品类型培养细胞
Unit SizeEach
内容与储存
• 350 mL 裂解混合物,在 15–30°C 下储存
• 3.5 mL 蛋白酶 K (50 µg/µL),在 –20°C 下储存

常见问题解答 (FAQ)

Can samples in TRIzol solution be run in the QuantiGene Plex assay?

No, samples in TRIzol solution cannot be run in the QuantiGene Plex assay. However, RNA purified from these samples can be run in the QuantiGene Plex assay.

Can I use the QuantiGene Plex assay with bacteria?

Although the kits were not originally validated for bacteria, we have guidelines for preparing bacterial samples for QuantiGene Plex assays: http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf

Do you offer QuantiGene Plex assay custom services where I can send my samples for you to process?

Yes, please reach out to your Technical Sales Specialist for more information or fill out this form: https://www.thermofisher.com/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html

Can I detect genomic DNA using the QuantiGene Plex assay?

Under the hybridization conditions that are used for the QuantiGene Plex assay, genomic DNA remains double-stranded. Therefore, it is not possible for the probes to bind to DNA. The signal from the probe sets comes only from RNA.

Can I use other lysis buffers with QuantiGene Plex assay samples?

We only recommend the use of QuantiGene Lysis Mixture for preparing cell and blood lysates. The Lysis Mixture has been optimized to provide stability of RNA in the lysates. In addition, QuantiGene Lysis Mixture provides the appropriate buffer conditions for the overnight hybridization.