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ViewRNA™ Fast Red 1-Plex Kit, 24 Assays - FAQs

查看更多产品信息 ViewRNA™ Colorimetric Tissue 1-Plex Kits - FAQs (QVT4430C, QVT4420C, QVT4410C, QVT0420C, QVT0430C, QVT0410C)

5 个常见问题解答

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer an alternative for the discontinued ViewRNA ISH Tissue Assay kits (2-plex) (Cat. No. QVT0012 and QVT0013)?

Currently, we offer the ViewRNA Colorimetric Tissue Assays in three formats:

- ViewRNA Colorimetric Tissue Core Kit (Cat. No. QVT0400) and  Individual modules for Fast Red (Cat. No. QVT0410), Fast Blue (Cat. No. QVT0420) and DAB (Cat. No. QVT0430) signal development
- ViewRNA Colorimetric Tissue 1-Plex Kits (Cat. No. QVT0420C, QVT4420C, QVT0410C, QVT4410C, QVT0430C, QVT4430C)
- ViewRNA Colorimetric Tissue 2-Plex Kits (Cat. No. QVT0433C, QVT4433C, QVT0412C, QVT4412C, QVT0422C, QVT4422C)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.