Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.
AuthorsRaffa P, Scattolini V, Gerli MFM, Perin S, Cui M, De Coppi P, Elvassore N, Caccin P, Luni C, Urciuolo A
JournalStem Cells Transl Med
PubMed ID32578968
'Skeletal muscle decellularization allows the generation of natural scaffolds that retain the extracellular matrix (ECM) mechanical integrity, biological activity, and three-dimensional (3D) architecture of the native tissue. Recent reports showed that in vivo implantation of decellularized muscles supports muscle regeneration in volumetric muscle loss models, including nervous system and neuromuscular ... More
In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure.
AuthorsArnette C, Koetsier JL, Hoover P, Getsios S, Green KJ
JournalMethods Enzymol
PubMed ID26778564
'Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D ... More
Low-level arsenic causes proteotoxic stress and not oxidative stress.
AuthorsDodson M, de la Vega MR, Harder B, Castro-Portuguez R, Rodrigues SD, Wong PK, Chapman E, Zhang DD
JournalToxicol Appl Pharmacol
PubMed ID29408041
'Prolonged exposure to arsenic has been shown to increase the risk of developing a number of diseases, including cancer and type II diabetes. Arsenic is present throughout the environment in its inorganic forms, and the level of exposure varies greatly by geographical location. The current recommended maximum level of arsenic ... More
Erratum: Long-range self-organization of cytoskeletal myosin II filament stacks.
AuthorsHu S, Dasbiswas K, Guo Z, Tee YH, Thiagarajan V, Hersen P, Chew TL, Safran SA, Zaidel-Bar R, Bershadsky AD
JournalNat Cell Biol
PubMed ID28248306
Cholesterol crystals increase vascular permeability by inactivating SHP2 and disrupting adherens junctions.
AuthorsMani AM, Chattopadhyay R, Singh NK, Rao GN
JournalFree Radic Biol Med
PubMed ID29782988
To understand the adverse effects of cholesterol crystals on vascular homeostasis, we have studied their effects on endothelial barrier function. Cholesterol crystals increased endothelial barrier permeability in a dose and time dependent manner. In addition, cholesterol crystals induced tyrosine phosphorylation of VE-cadherin and a-catenin, disrupting endothelial AJ and its barrier ... More
Expression of (pro)renin receptor and its effect on endothelial cell proliferation in infantile hemangioma.
Authorsvan Schaijik B, Tan ST, Marsh RW, Itinteang T
JournalPediatr Res
PubMed ID31091531
Propranolol is the preferred treatment for problematic proliferating infantile hemangioma (IH) by targeting the renin-angiotensin system (RAS) expressed by IH endothelium. (Pro)renin receptor (PRR) is a major component of the RAS associated with the canonical wnt signaling pathway. We proposed that activation of PRR by renin causes proliferation of IH. ... More
Proliferating infantile hemangioma promotes a-fetoprotein production by HepG2 cells.
Authorsvan Schaijik B, de Jongh J, Marsh RW, Munro M, Itinteang T, Tan ST