Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expressionRead more
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Catalog Number
Quantity
R0533
200 μL
R0534
5 x 200 μL
R0531
1 mL
R0532
5 x 1 mL
4 Options
Catalog number R0533
Price (CNY)
944.00
Each
Add to cart
Quantity:
200 μL
Price (CNY)
944.00
Each
Add to cart
Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.
Features of TurboFect Transfection Reagent:
• High transfection efficiency of a wide variety of cell types • No optimization required for most cell lines • Excellent transfection efficiency in the presence or absence of serum • Minimal cytotoxicity • Ready-to-use—no need to reconstitute, dilute or manipulate
Applications of TurboFect Transfection Reagent : • Stable and transient transfection or co-transfection of DNA or RNA • Experiments with adherent or suspension cells, differentiated or undifferentiated cells • Gene expression studies and assays involving GFP or luciferase reporters
TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineTurboFect™
Quantity200 μL
Sufficient For100 Transfections
Transfection TechniqueLipid-Based Transfection
TypeTransfection Reagent
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
Frequently asked questions (FAQs)
What is the stability of TurboFECT after being frozen?
TurboFect is a sterile solution of a proprietary cationic polymer in water. Its performance is not affected by a few freeze-thaw cycles. If TurboFECT arrives frozen or is accidentally stored at -20 degrees C, simply thaw and mix it thoroughly before use. For long-term storage, keep TurboFECT at 4 degrees C as recommended.
What are the advantages of using TurboFect Transfection Reagent (Cat. No. R0533) over other transfection reagents with the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186)?
The Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) luciferase vectors were optimized with TurboFect. However, it should be feasible to replace TurboFect with another transfection reagent if your cells tolerate the transfection reagent better and give you acceptable transfection rates.