Modulation by Syk of Bcl-2, calcium and the calpain-calpastatin proteolytic system in human breast cancer cells.
AuthorsFei B, Yu S, Geahlen RL,
Journal
PubMed ID23684705
'Syk is a 72kDa non-receptor tyrosine kinase that is best characterized in hematopoietic cells. While Syk is pro-tumorigenic in some cancer cell types, it also has been reported as a negative regulator of metastatic cell growth in others. An examination of the RelA (p65) subunit of NF-?B expressed in MCF7 ... More
Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors.
AuthorsIeda M, Fu JD, Delgado-Olguin P, Vedantham V, Hayashi Y, Bruneau BG, Srivastava D,
JournalCell
PubMed ID20691899
'The reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) raises the possibility that a somatic cell could be reprogrammed to an alternative differentiated fate without first becoming a stem/progenitor cell. A large pool of fibroblasts exists in the postnatal heart, yet no single ' ... More
Induction of human cardiomyocyte-like cells from fibroblasts by defined factors.
'Heart disease remains a leading cause of death worldwide. Owing to the limited regenerative capacity of heart tissue, cardiac regenerative therapy has emerged as an attractive approach. Direct reprogramming of human cardiac fibroblasts (HCFs) into cardiomyocytes may hold great potential for this purpose. We reported previously that induced cardiomyocyte-like cells ... More
MKP3 eliminates depolarization-dependent neurotransmitter release through downregulation of L-type calcium channel Cav1.2 expression.
AuthorsMortensen OV,
JournalCell Calcium
PubMed ID23337371
'Release of neurotransmitters is a fundamental and regulated process that is essential for normal brain functioning. Regulation of this process is potentially important for any neuronal process, and disruption of the release process may contribute to the pathophysiology associated with psychiatric diseases. In this work it is shown that expression ... More
Neprilysin deficiency protects against fat-induced insulin secretory dysfunction by maintaining calcium influx.
AuthorsZraika S, Koh DS, Barrow BM, Lu B, Kahn SE, Andrikopoulos S,
JournalDiabetes
PubMed ID23328128
'Neprilysin contributes to free fatty acid (FFA)-induced cellular dysfunction in nonislet tissues in type 2 diabetes. Here, we show for the first time that with prolonged FFA exposure, islet neprilysin is upregulated and this is associated with reduced insulin pre-mRNA and ATP levels, oxidative/nitrative stress, impaired potassium and calcium channel ... More
Accumulation of GABAergic Neurons, Causing a Focal Ambient GABA Gradient, and Downregulation of KCC2 Are Induced During Microgyrus Formation in a Mouse Model of Polymicrogyria.
AuthorsWang T, Kumada T, Morishima T, Iwata S, Kaneko T, Yanagawa Y, Yoshida S, Fukuda A,
JournalCereb Cortex
PubMed ID23246779
'Although focal cortical malformations are considered neuronal migration disorders, their formation mechanisms remain unknown. We addressed how the ?-aminobutyric acid (GABA)ergic system affects the GABAergic and glutamatergic neuronal migration underlying such malformations. A focal freeze-lesion (FFL) of the postnatal day zero (P0) glutamic acid decarboxylase-green fluorescent protein knock-in mouse neocortex ... More
Development and in vitro characterization of ratiometric and intensity-based fluorescent ion sensors.
AuthorsCooley LA, Martin VV, Gee KR,
JournalMethods Mol Biol
PubMed ID23494377
Fluorescent ion sensors are quite valuable in experimental biology. The development of new sensor molecules requires determination of spectral properties (absorption bands, fluorescence excitation, and emission maxima) in order to characterize the type of optical response to the target ion. This optical response type and magnitude are used, in combination ... More
Inositol 1,4,5-trisphosphate receptor regulates replication, differentiation, infectivity and virulence of the parasitic protist Trypanosoma cruzi.
AuthorsHashimoto M, Enomoto M, Morales J, Kurebayashi N, Sakurai T, Hashimoto T, Nara T, Mikoshiba K,
JournalMol Microbiol
PubMed ID23320762
In animals, inositol 1,4,5-trisphosphate receptors (IP3 Rs) are ion channels that play a pivotal role in many biological processes by mediating Ca(2+) release from the endoplasmic reticulum. Here, we report the identification and characterization of a novel IP3 R in the parasitic protist, Trypanosoma cruzi, the pathogen responsible for Chagas ... More
Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro.
AuthorsQian L, Berry EC, Fu JD, Ieda M, Srivastava D,
Journal
PubMed ID23722259
Cardiac fibroblasts can be reprogrammed to cardiomyocyte-like cells by the introduction of three transcription factors: Gata4, Mef2c and Tbx5 (collectively referred to here as GMT). Resident cardiac fibroblasts can be converted in vivo into induced cardiomyocyte-like cells (iCMs) that closely resemble endogenous cardiomyocytes and electrically integrate with the host myocardium. ... More
Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury.
Authors
JournalCell
PubMed ID30712871
TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility.
Authors
JournalJ Am Soc Nephrol
PubMed ID31416818
Upward synaptic scaling is dependent on neurotransmission rather than spiking.
Authors
JournalNat Commun
PubMed ID25751516
Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming.
Authors
JournalNat Commun
PubMed ID30787297
Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression.
Authors
JournalInt J Mol Sci
PubMed ID28825623
Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen.
Authors
JournalJ Exp Med
PubMed ID32697822
Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions.
Authors
JournalStem Cell Reports
PubMed ID26626177
Direct In Vivo Reprogramming with Sendai Virus Vectors Improves Cardiac Function after Myocardial Infarction.
Authors
JournalCell Stem Cell
PubMed ID29276141
CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes.
Authors
JournalNeuron
PubMed ID32485136
PKD2/polycystin-2 induces autophagy by forming a complex with BECN1.