Interpretation and optimization of absorbance and fluorescence signals from voltage-sensitive dyes.
AuthorsChang PY, Jackson MB
JournalJ Membr Biol
PubMed ID14724747
'Voltage-sensitive dyes produce absorbance and fluorescence changes that can be used to image voltage. The present study develops a systematic approach to the optimization of these signals. A mathematical analysis assesses the dye optical density ( OD) that optimizes the signal-to-noise ratio in absorbance and fluorescence measurements. The signal-to-noise ratio ... More
Functionally independent columns of rat somatosensory barrel cortex revealed with voltage-sensitive dye imaging.
AuthorsPetersen CC, Sakmann B
JournalJ Neurosci
PubMed ID11606632
'Whisker movement is somatotopically represented in rodent neocortex by electrical activity in clearly defined barrels, which can be visualized in living brain slices. The functional architecture of this part of the cortex can thus be mapped in vitro with respect to its physiological input and compared with its anatomical architecture. ... More
Optical monitoring of activity of many neurons in invertebrate ganglia during behaviors.
AuthorsWu JY, London JA, Zecevic D, Höpp HP, Cohen LB, Xiao C
JournalExperientia
PubMed ID3286282
'Optical methods for monitoring neuron activity were developed because these methods lend themselves to simultaneous multiple-site measurements. With the use of new voltage-sensitive dyes, the dye-related pharmacology and photodynamic damage appear to be relatively unimportant. Using multiple-site measurements made with a 124-element photodiode array, we estimated that approximately 30 of ... More
Optical recording of voltage changes in nerve terminals and in fine neuronal processes.
AuthorsSalzberg BM
JournalAnnu Rev Physiol
PubMed ID2653193
Optical imaging of neuronal activity.
AuthorsGrinvald A, Frostig RD, Lieke E, Hildesheim R
JournalPhysiol Rev
PubMed ID3054949
Voltage-sensitive dyes and functional activity in the olfactory pathway.
AuthorsCinelli AR, Kauer JS
JournalAnnu Rev Neurosci
PubMed ID1575446
Optical recording of action potential propagation in demyelinated frog nerve.
Conduction in focally demyelinated frog nerves has been measured optically using potential-sensitive dyes. Absorption changes were recorded with an array of photodiodes positioned in the image plane of a microscope. Both the amplitude and conduction velocity of the optical signals decreased in the demyelinated region. Conduction was improved after exposure ... More
Optical recording and information theoretic analysis of Aplysia gill-withdrawal reflex.
AuthorsShiono S, Nakashima M, Yamada S, Matsumoto K
JournalJpn J Physiol
PubMed ID8271513
A new experimental setup has been devised to perform Aplysia optical recording, the firing of the siphon sensory neurons being precisely controlled. Significant crosscorrelations between the siphon sensory neurons and optically detected neurons were obtained by our information theory-based analysis, and we discussed possible roles of the detected neurons in ... More
Imaging membrane potential with voltage-sensitive dyes.
AuthorsZochowski M, Wachowiak M, Falk CX, Cohen LB, Lam YW, Antic S, Zecevic D
JournalBiol Bull
PubMed ID10707808
Membrane potential can be measured optically using a variety of molecular probes. These measurements can be useful in studying function at the level of an individual cell, for determining how groups of neurons generate a behavior, and for studying the correlated behavior of populations of neurons. Examples of the three ... More
Simultaneous optical recording of activity from many neurons during feeding in Navanax.
AuthorsLondon JA, Zecevic D, Cohen LB
JournalJ Neurosci
PubMed ID2435862
Optical methods for monitoring changes in membrane potential have been used to measure action potential activity in the buccal ganglion of an opisthobranch mollusc, Navanax inermis, while the animal was feeding. During feeding activity was detected in 10-15% of the approximately 200 neurons present in the ganglion. Control experiments carried ... More
Entorhinal-hippocampal interactions revealed by real-time imaging.
AuthorsIijima T, Witter MP, Ichikawa M, Tominaga T, Kajiwara R, Matsumoto G
JournalScience
PubMed ID8638163
The entorhinal cortex provides the major cortical input to the hippocampus, and both structures have been implicated in memory processes. The dynamics of neuronal circuits in the entorhinal-hippocampal system were studied in slices by optical imaging with high spatial and temporal resolution. Reverberation of neural activity was detected in the ... More
Ca2+- and K+-dependent communication between central nervous system myelinated axons and oligodendrocytes revealed by voltage-sensitive dyes.
AuthorsLev-Ram V, Grinvald A
JournalProc Natl Acad Sci U S A
PubMed ID2428038
The interactions between myelinated axons and surrounding glia cells, in rat optic nerve, were investigated by optical recording with voltage-sensitive dyes. Electrical stimulation of the nerve evoked an optical signal revealing two clearly distinct components: a fast propagating component, corresponding to the compound action potential, and a prominent slow component. ... More
Optical analysis of acute spontaneous epileptiform discharges in the in vivo rat cerebral cortex.
AuthorsMiyakawa N, Yazawa I, Sasaki S, Momose-Sato Y, Sato K
JournalNeuroimage
PubMed ID12667839
We examined the spatiotemporal patterns of spontaneous epileptiform activity observed in the in vivo rat cerebral cortex using an optical recording technique of detecting transmembrane voltage changes. The surface of the cerebral cortex was exposed under anesthesia and stained with a fluorescent voltage-sensitive dye, RH414. Acute spontaneous epileptiform discharges were ... More
Optical recording of the electrical activity of synaptically interacting Aplysia neurons in culture using potentiometric probes.
We used multiple-site optical recording methods, in conjunction with impermeant molecular probes of the cell membrane potential, to record the electrical activity of model neural circuits in vitro. Our system consisted of co-cultured pairs of left upper quadrant neurons from the abdominal ganglion of the marine gastropod Aplysia. These neurons ... More
Optical detection of neuromodulatory effects of conditioned taste aversion in the pond snail Lymnaea stagnalis.
AuthorsKojima S, Hosono T, Fujito Y, Ito E
JournalJ Neurobiol
PubMed ID11598919
Multiple site optical recording was used to analyze the neural activity changes caused by conditioned taste aversion (CTA) training in the pond snail Lymnaea stagnalis. In response to electrical stimulation of the median lip nerve, which transmits chemosensory signals of appetitive taste to the central nervous system, we optically detected ... More
A nonlinear electrostatic potential change in the T-system of skeletal muscle detected under passive recording conditions using potentiometric dyes.
AuthorsHeiny JA, Jong DS
JournalJ Gen Physiol
PubMed ID2299329
Voltage-sensing dyes were used to examine the electrical behavior of the T-system under passive recording conditions similar to those commonly used to detect charge movement. These conditions are designed to eliminate all ionic currents and render the T-system potential linear with respect to the command potential applied at the surface ... More
Influence of substrate on the distribution of calcium channels in identified leech neurons in culture.
AuthorsRoss WN, Aréchiga H, Nicholls JG
JournalProc Natl Acad Sci U S A
PubMed ID2453887
The Retzius neuron from the leech, growing in culture on the plant lectin concanavalin A as substrate, produces broad flat growth cones and thick bundles of processes. The same cell extends fine straight processes with numerous branches when grown on a laminin-like substrate extracted from leech central nervous system extracellular ... More
Optical recording of calcium and voltage transients following impulses in cell bodies and processes of identified leech neurons in culture.
AuthorsRoss WN, Arechiga H, Nicholls JG
JournalJ Neurosci
PubMed ID2447247
Optical methods were used to examine the spread of electrical potentials and the distribution and time course of calcium transients in individual identified nerve cells isolated from the leech. A photodiode array detected voltage transients by measuring absorbance changes of cells stained with the voltage-sensitive dye RH155 added to the ... More
Long-term optical recording of patterns of electrical activity in ensembles of cultured Aplysia neurons.
AuthorsParsons TD, Salzberg BM, Obaid AL, Raccuia-Behling F, Kleinfeld D
JournalJ Neurophysiol
PubMed ID1919674
1. Left upper quadrant (LUQ) cells isolated from the abdominal ganglion of Aplysia were maintained in culture to study how the cellular and synaptic properties of individual neurons contribute to the generation of patterns of electrical activity by neuronal ensembles. 2. Conventional microelectrodes were used to examine the spiking characteristics ... More
Glutamate release increases during mossy-CA3 LTP but not during Schaffer-CA1 LTP.
AuthorsKawamura Y, Manita S, Nakamura T, Inoue M, Kudo Y, Miyakawa H
JournalEur J Neurosci
PubMed ID15066155
Abstract It is still a matter of dispute whether the expression of hippocampal long-term potentiation (LTP) is due to enhanced transmitter release or enhanced postsynaptic sensitivity. Recently we developed a novel method to monitor synaptically released glutamate. In this method, brain slice preparations are stained with a voltage-sensitive dye RH155 ... More
Consistency in nervous systems: trial-to-trial and animal-to-animal variations in the responses to repeated applications of a sensory stimulus in Aplysia.
What is the internal noise in a nervous system? We studied this question by determining the trial-to-trial consistency of the neuronal response in the abdominal ganglion of Aplysia californica. Because our voltage-sensitive dye recordings detected the spike activity from a large fraction of the neurons in the ganglion, these results ... More
High-speed optical imaging of afferent flow through rat olfactory bulb slices: voltage-sensitive dye signals reveal periglomerular cell activity.
AuthorsSenseman DM
JournalJ Neurosci
PubMed ID8613798
Fast, multiple-site optical recording and video imaging techniques were combined to visualize the olfactory processing stream as it flowed through rat olfactory bulb slices stained with the voltage-sensitive dye RH155. A 464 element photodiode detector array was used to record the voltage-sensitive dye signals. Focal electrical stimulation of the olfactory ... More
Optical signals from neurons with internally applied voltage-sensitive dyes.
AuthorsAntic S, Zecevic D
JournalJ Neurosci
PubMed ID7869106
We carried out experiments to monitor optically the generation and spread of action potentials and subthreshold potentials in the processes of individual neurons in ganglia of the snail, Helix aspersa. The neurons were selectively stained by intracellular pressure injection of voltage-sensitive dyes. Optical signals were detected by a system for ... More