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Quant-iT™ RiboGreen RNA 定量试剂盒与 RiboGreen RNA 试剂、RediPlate™ 96 RiboGreen™ RNA 定量试剂盒
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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| 货号 | 数量 | 产品类型 | 定量范围 |
|---|---|---|---|
| R11491 | 1 mL | Quant-iT RiboGreen RNA 试剂 | 1 至 200 ng |
| R11490 | 1 kit | Quant-iT RiboGreen RNA 试剂盒 | 1 至 200 ng |
| R32700 | 1 kit | RediPlate 96 RiboGreen RNA 试剂盒 | 3 至 200 ng |
货号 R11491
价格（CNY)
7,293.00
Each
数量:
1 mL
产品类型:
Quant-iT RiboGreen RNA 试剂
定量范围:
1 至 200 ng
价格（CNY)
7,293.00
Each
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
Applications
Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.
仅供科研使用。不可用于诊断程序。
规格
激发/发射500/525
适用于（设备）微孔板读数仪
反应次数200-2,000 次试管测定，使用 2 mL 测定体积
产品线Quant-iT、RIBOGREEN
产品类型Quant-iT RiboGreen RNA 试剂
定量范围1 至 200 ng
数量1 mL
运输条件室温
检测方法荧光
Unit SizeEach