Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy.
AuthorsDavidson SM, Duchen MR,
JournalMethods Mol Biol
PubMed ID22057570
The concentration of calcium ions in the mitochondria has a profound impact on mitochondrial function, modulating respiratory activity at physiological concentrations, while causing lethal damage during calcium overload. The ... More
Predicting and avoiding subcellular compartmentalization artifacts arising from acetoxymethyl ester calcium imaging probes. The case of fluo-3 AM and a general account of the phenomenon including a problem avoidance chart.
AuthorsThompson K, Dockery P, Horobin RW,
JournalBiotech Histochem
PubMed ID22984940
Stimulated by difficulties experienced when using fluo-3 AM, we developed a general mechanistic model to aid understanding and practical application of calcium probes applied as acetoxymethyl (AM) esters. Several practical issues previously overlooked or under-emphasized are considered by this model. First, some AM ester probes are ... More
Azumolene inhibits a component of store-operated calcium entry coupled to the skeletal muscle ryanodine receptor.
AuthorsZhao X, Weisleder N, Han X, Pan Z, Parness J, Brotto M, Ma J,
JournalJ Biol Chem
PubMed ID16945924
Dantrolene reduces the elevated myoplasmic Ca(2+) generated during malignant hyperthermia, a pharmacogenetic crisis triggered by volatile anesthetics. Although specific binding of dantrolene to the type 1 ryanodine receptor (RyR1), the Ca(2+) release channel of skeletal muscle sarcoplasmic reticulum, has been demonstrated, there is little evidence for direct dantrolene inhibition of ... More
PINK1-associated Parkinson's disease is caused by neuronal vulnerability to calcium-induced cell death.
AuthorsGandhi S, Wood-Kaczmar A, Yao Z, Plun-Favreau H, Deas E, Klupsch K, Downward J, Latchman DS, Tabrizi SJ, Wood NW, Duchen MR, Abramov AY,
JournalMol Cell
PubMed ID19285945
Mutations in PINK1 cause autosomal recessive Parkinson's disease. PINK1 is a mitochondrial kinase of unknown function. We investigated calcium homeostasis and mitochondrial function in PINK1-deficient mammalian neurons. We demonstrate physiologically that PINK1 regulates calcium efflux from the mitochondria via the mitochondrial Na(+)/Ca(2+) exchanger. PINK1 deficiency causes mitochondrial accumulation of calcium, ... More
Quantitative estimate of mitochondrial [Ca2+] in stimulated motor nerve terminals.
AuthorsDavid G, Talbot J, Barrett EF
JournalCell Calcium
PubMed ID12600806
Peak values reported for mitochondrial matrix [Ca(2+)] following stimulation have ranged from micromolar to near-millimolar in various cells. Measurements using fluorescent indicators have traditionally used high-affinity dyes such as rhod-2, whose fluorescence would be expected to saturate if matrix [Ca(2+)] approaches millimolar levels. To avoid this potential problem, we loaded ... More