Development of fluorescence-based selective assays for serine/threonine and tyrosine phosphatases.
AuthorsPastula C, Johnson I, Beechem JM, Patton WF
JournalComb Chem High Throughput Screen
PubMed ID12769677
A number of aromatic substrates were evaluated for their ability to detect tyrosine phosphatase and serine/threonine phosphatase activity. Results demonstrated that the fluorinated coumarin DiFMUP is the most sensitive substrate for detecting LAR and PP-2A activity. Using this substrate, selective high-throughput screening assays for serine/threonine and tyrosine phosphatases were developed. ... More