罗丹明123，25 mg

如果使用我们的FluoVolt 膜电势试剂盒（货号F10488），该试剂盒包含一种背景抑制剂，可改善这一问题。对于其他指标剂，可以考虑使用BackDrop 背景抑制剂（货号R37603、B10511和B10512）。

在周围电场的作用下结构变化的分子可用作检测瞬时（毫秒级）电位变化的快反应探针。慢反应染料则会进入去极化细胞，结合蛋白或膜。增强去极化会造成额外的染料流入，增强荧光强度；过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像，或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

膜电位指示剂选择指南请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html）。

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm^-1 M^-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.