罗丹明123，25 mg
罗丹明123，25 mg
引用和文献 (715)
Invitrogen™

罗丹明123，25 mg

罗丹明123是一种细胞膜通透性阳离子绿荧光染料可被活性线粒体轻松吸收，且无细胞毒性。该产品已用于检测凋亡细胞群中的线粒体膜电位。了解更多信息
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货号数量
R30225 mg
货号 R302
价格（CNY)
1,248.00
飞享价
Ends: 31-Dec-2025
1,691.00
共减 443.00 (26%)
Each
添加至购物车
数量:
25 mg
价格（CNY)
1,248.00
飞享价
Ends: 31-Dec-2025
1,691.00
共减 443.00 (26%)
Each
添加至购物车
罗丹明123是一种细胞膜通透性阳离子绿荧光染料可被活性线粒体轻松吸收，且无细胞毒性。该产品已用于检测凋亡细胞群中的线粒体膜电位。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量25 mg
运输条件室温
子细胞定位线粒体
颜色绿色
适用于（设备）荧光显微镜
产品类型罗丹明 123
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时，看到神经元周围出现了较高的背景，如何降低背景干扰？

如果使用我们的FluoVolt 膜电势试剂盒（货号F10488），该试剂盒包含一种背景抑制剂，可改善这一问题。对于其他指标剂，可以考虑使用BackDrop 背景抑制剂（货号R37603、B10511和B10512）。

快反应膜电位探针和慢反应膜电位探针有什么区别？

在周围电场的作用下结构变化的分子可用作检测瞬时（毫秒级）电位变化的快反应探针。慢反应染料则会进入去极化细胞，结合蛋白或膜。增强去极化会造成额外的染料流入，增强荧光强度；过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像，或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂？我该如何根据自己的试验选择？

膜电位指示剂选择指南请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html）。

What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye Excitation Emission EC
Rhodamine B 568 583 88,000
Rhodamine 123 507 529 101,000
Rhodamine 110 499 521 92,000
Rhodamine 6G 528 551 105,000
XRITC 572 596 92,000


Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all 罗丹明123，25 mg FAQs

引用和文献 (715)

引用和文献
Abstract
The mitochondrial death/life regulator in apoptosis and necrosis.
Authors:Kroemer G,Dallaporta B,Resche-Rigon M
Journal:Annual review of physiology
PubMed ID:9558479
Both physiological cell death (apoptosis) and, in some cases, accidental cell death (necrosis) involve a two-step process. At a first level, numerous physiological and some pathological stimuli trigger an increase in mitochondrial membrane permeability. The mitochondria release apoptogenic factors through the outer membrane and dissipate the electrochemical gradient of the ... More
2,4 Dinitrophenol-uncoupling effect on delta psi in living hepatocytes depends on reducing-equivalent supply.
Authors:Sibille B, Ronot X, Filippi C, Nogueira V, Keriel C, Leverve X
Journal:Cytometry
PubMed ID:9627223
Mitochondrial uncouplers, such as 2,4 dinitrophenol (DNP), increase the cellular respiration by decreasing mitochondrial membrane potential (delta psi). We show that this respiratory effect can be transient or even prevented in isolated liver cells depending on the exogenous substrate used (dihydroxyacetone vs. octanoate or proline). Moreover the decrease in ATP/ADP ... More
Is rhodamine 123 an appropriate fluorescent probe to assess P-glycoprotein mediated multidrug resistance in vinblastine-resistant CHO cells?
Authors:Pétriz J, O'Connor JE, Carmona M, García-López J
Journal:Anal Cell Pathol
PubMed ID:9354229
Cellular drug resistance, which involves several mechanisms such as P-glycoprotein (P-gp) overexpression, kinetic and metabolic quiescence, or the increase in the intracellular levels of glutathione, limits the effectiveness of cancer treatment. It has been reported that functional assessment of the cationic dye rhodamin 123 (Rho123) efflux reveals accurately the drug-resistant ... More
Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential.
Authors:Maftah A, Ratinaud MH, Dumas M, Bonté F, Meybeck A, Julien R
Journal:Mech Ageing Dev
PubMed ID:7745994
Mitochondria dysfunction is considered to be a major cause of the modifications that occur during cell ageing. For this reason, cardiolipin, a suitable marker of the chondriome, as well as the mitochondrial transmembrane potential were examined in keratinocytes obtained from 9- to 75-year-old women. The study was carried out by ... More
Flow cytometric functional analysis of multidrug resistance by Fluo-3: a comparison with rhodamine-123.
Authors:Koizumi S, Konishi M, Ichihara T, Wada H, Matsukawa H, Goi K, Mizutani S
Journal:Eur J Cancer
PubMed ID:7488425
Using four cell lines including drug-sensitive K562/Parent cells, P-glycoprotein (Pgp)-mediated multidrug resistant (MDR) K562/VCR, K562/ADR and revertant K562/ADR-R cells, two fluorescent agents, Fluo-3 and rhodamine-123 (Rh-123), were compared as indicators in a functional assay of MDR. Cells were incubated with 4 microM Fluo-3 or 1 microM Rh-123 for 45 min ... More
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