I am using the EZQ Protein Quantitation Kit and the filter paper has curled up during drying. What should I do?
It is not unusual for the filter paper to bend and warp on drying. It is very important that the paper be flat when scanning or the signal will be uneven and give very inaccurate quantitation values. If this is a problem, wet the paper in water and scan it while it is wet.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I am using the EZQ Protein Quantitation Kit and after staining, I notice some large spots or marks on the paper. Why is this?
Protein on the skin (e.g. keratin) will transfer to the filter paper and be stained with the EZQ Protein Quantitation Reagent. We recommend handling the filter paper with tweezers and cleaning the staining dish and tweezers before use to minimize marks.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I spotted my protein samples onto the EZQ protein assay filter paper and I do not have time to complete the assay? Can I stop the procedure and finish later?
Yes, once the samples have dried onto the filter paper, the filter paper can be stored and stained at a later date. After the staining procedure is complete, the signal is very stable, so the paper can be dried again and scanned at a later date as well. The paper can be scanned dry or after dipping it in water. If storing the paper before staining, we recommend storing it in a plastic bag to prevent contamination that could affect the staining pattern.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I ran out of filter paper for the EZQ Protein Quantitation Kit. Can I just cut any filter paper to fit inside the microplate?
No. The filter paper that is supplied with the EZQ Protein Quantitation Kit is a very specific filter paper and the identity is proprietary. Most lab filter papers will not provide similar results as the paper provided in the kit, so substitutions are not recommended.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?
You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
