Presynaptic calcium measurements at physiological temperatures using a new class of dextran-conjugated indicators.
AuthorsBeierlein M, Gee KR, Martin VV, Regehr WG
JournalJ Neurophysiol
PubMed ID15212445
Presynaptic calcium (Ca(pre)) has been studied extensively because of its role in triggering and modulating neurotransmitter release. Although calcium regulation and calcium-driven processes can be strongly temperature dependent, technical difficulties have limited most studies of Ca(pre) to temperatures well below the physiological range. Here we assessed the use of membrane-permeant ... More