Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment.
AuthorsColvin RA, Laskowski M, Fontaine CP,
JournalBrain Res
PubMed ID16581038
'Zinquin (Zn(2+) selective fluorophore), when used to visualize intracellular Zn(2+), typically shows brightly fluorescent perinuclear endosome-like structures, presumably identifying Zn(2+) containing organelles. In this study, zinquin identified numerous and widespread sites of Zn(2+) compartmentalization in primary cultures of embryonic rat cortical neurons. Nuclear fluorescence, however, was absent. We labeled neuronal ... More
Metals in neurobiology: probing their chemistry and biology with molecular imaging.
AuthorsQue EL, Domaille DW, Chang CJ,
JournalChem Rev
PubMed ID18426241
'In this review, we will provide a brief overview of the field of transition metals in neurobiology, focusing on the contributions of d-block metals zinc, iron, and copper to neurophysiology, aging, and neuropathology, as well as progress in the development of molecular probes for visualizing zinc, iron, and copper ion ... More
Zinc-dependent multi-conductance channel activity in mitochondria isolated from ischemic brain.
AuthorsBonanni L, Chachar M, Jover-Mengual T, Li H, Jones A, Yokota H, Ofengeim D, Flannery RJ, Miyawaki T, Cho CH, Polster BM, Pypaert M, Hardwick JM, Sensi SL, Zukin RS, Jonas EA,
JournalJ Neurosci
PubMed ID16793892
'Transient global ischemia is a neuronal insult that induces delayed cell death. A hallmark event in the early post-ischemic period is enhanced permeability of mitochondrial membranes. The precise mechanisms by which mitochondrial function is disrupted are, as yet, unclear. Here we show that global ischemia promotes alterations in mitochondrial membrane ... More
A new mitochondrial fluorescent zinc sensor.
AuthorsSensi SL, Ton-That D, Weiss JH, Rothe A, Gee KR
JournalCell Calcium
PubMed ID12887975
A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that ... More
The thiol-modifying agent N-ethylmaleimide elevates the cytosolic concentration of free Zn(2+) but not of Ca(2+) in murine cortical neurons.
AuthorsGibon J, Tu P, Frazzini V, Sensi SL, Bouron A,
JournalCell Calcium
PubMed ID20667413
The membrane permeant alkylating agent N-ethylmaleimide (NEM) regulates numerous biological processes by reacting with thiol groups. Among other actions, NEM influences the cytosolic concentration of free Ca(2+) ([Ca(2+)]i). Depending on the cell type and the concentration used, NEM can promote the release of Ca(2+), affect its extrusion, stimulate or block ... More
Synthetic fluorescent sensors for studying the cell biology of metals.
AuthorsDomaille DW, Que EL, Chang CJ,
JournalNat Chem Biol
PubMed ID18277978
Metals are essential for sustaining all forms of life, but alterations in their cellular homeostasis are connected to severe human disorders, including cancer, diabetes and neurodegenerative diseases. Fluorescent small molecules that respond to metal ions in the cell with appropriate selectivity and sensitivity offer the ability to probe physiological and ... More
Antiviral activity of the zinc ionophores pyrithione and hinokitiol against picornavirus infections.
AuthorsKrenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJ, Seipelt J,
JournalJ Virol
PubMed ID18922875
We have discovered two metal ion binding compounds, pyrithione (PT) and hinokitiol (HK), that efficiently inhibit human rhinovirus, coxsackievirus, and mengovirus multiplication. Early stages of virus infection are unaffected by these compounds. However, the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by the rhinoviral 2A protease was abolished ... More
Modulation of mitochondrial function by endogenous Zn2+ pools.
Recent evidence suggests that intracellular Zn(2+) accumulation contributes to the neuronal injury that occurs in epilepsy or ischemia in certain brain regions, including hippocampus, amygdala, and cortex. Although most attention has been given to the vesicular Zn(2+) that is released into the synaptic space and may gain entry to postsynaptic ... More