Actin Dynamics Regulated by the Balance of Neuronal Wiskott-Aldrich Syndrome Protein (N-WASP) and Cofilin Activities Determines the Biphasic Response of Glucose-induced Insulin Secretion.
AuthorsUenishi E, Shibasaki T, Takahashi H, Seki C, Hamaguchi H, Yasuda T, Tatebe M, Oiso Y, Takenawa T, Seino S,
Journal
PubMed ID23867458
'Actin dynamics in pancreatic ß-cells is involved in insulin secretion. However, the molecular mechanisms of the regulation of actin dynamics by intracellular signals in pancreatic ß-cells and its role in phasic insulin secretion are largely unknown. In this study, we elucidate the regulation of actin dynamics by neuronal Wiskott-Aldrich syndrome ... More
Translational control of Nrf2 within the open reading frame.
AuthorsPerez-Leal O, Barrero CA, Merali S,
Journal
PubMed ID23806685
'Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) is a transcription factor that is essential for the regulation of an effective antioxidant and detoxifying response. The regulation of its activity can occur at transcription, translation and post-translational levels. Evidence suggests that under environmental stress conditions, new synthesis of Nrf2 is required ... More
Neutrophil cathepsin G, but not elastase, induces aggregation of MCF-7 mammary carcinoma cells by a protease activity-dependent cell-oriented mechanism.
AuthorsYui S, Osawa Y, Ichisugi T, Morimoto-Kamata R,
Journal
PubMed ID24803743
'We previously found that a neutrophil serine protease, cathepsin G, weakens adherence to culture substrates and induces E-cadherin-dependent aggregation of MCF-7 human breast cancer cells through its protease activity. In this study, we examined whether aggregation is caused by degradation of adhesion molecules on the culture substrates or through an ... More
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3-ch-TOG onto mitotic spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule-binding ... More
Assessing telomere length using surface enhanced Raman scattering.
AuthorsZong S, Wang Z, Chen H, Cui Y,
Journal
PubMed ID25381775
Telomere length can provide valuable insight into telomeres and telomerase related diseases, including cancer. Here, we present a brand-new optical telomere length measurement protocol using surface enhanced Raman scattering (SERS). In this protocol, two single strand DNA are used as SERS probes. They are labeled with two different Raman molecules ... More
SERS-fluorescence joint spectral encoded magnetic nanoprobes for multiplex cancer cell separation.
AuthorsWang Z, Zong S, Chen H, Wang C, Xu S, Cui Y,
Journal
PubMed ID24862088
A new kind of cancer cell separation method is demonstrated, using surface-enhanced Raman scattering (SERS) and fluorescence dual-encoded magnetic nanoprobes. The designed nanoprobes can realize SERS-fluorescence joint spectral encoding (SFJSE) and greatly improve the multiplexing ability. The nanoprobes have four main components, that is, the magnetic core, SERS generator, fluorescent ... More
Photodynamic therapy with decacationic [60]fullerene monoadducts: effect of a light absorbing electron-donor antenna and micellar formulation.
AuthorsYin R, Wang M, Huang YY, Huang HC, Avci P, Chiang LY, Hamblin MR,
Journal
PubMed ID24333585
We report the synthesis and anticancer photodynamic properties of two new decacationic fullerene (LC14) and red light-harvesting antenna-fullerene conjugated monoadduct (LC15) derivatives. The antenna of LC15 was attached covalently to C60>with distance of only <3.0 ? to facilitate ultrafast intramolecular photoinduced-electron-transfer (for type-I photochemistry) and photon absorption at longer wavelengths. ... More
Comparative studies of cellular viability levels on 2D and 3D in vitro culture matrices.
AuthorsGargotti M, Lopez-Gonzalez U, Byrne HJ, Casey A
JournalCytotechnology
PubMed ID28924965
'In this study, the cellular viability and function of immortalized human cervical and dermal cells are monitored and compared in conventional 2D and two commercial 3D membranes, Collagen and Geltrex, of varying working concentration and volume. Viability was monitored with the aid of the Alamar Blue assay, cellular morphology was ... More
Improvement of Red Blood Cell Maturation
AuthorsKim SH, Lee EM, Han SY, Choi HS, Ryu KY, Baek EJ
JournalTissue Eng Part C Methods
PubMed ID30848173
'Despite recent studies on media additives, the low viability and dysplastic features of terminally mature erythroid cells are still major problems in enhancing erythroid cell yields' ... More
Quantitative measurements of early alphaviral replication dynamics in single cells reveals the basis for superinfection exclusion.
Authors
JournalCell Syst
PubMed ID33515490
Assessment of liposome disruption to quantify drug delivery in vitro.
AuthorsNogueira E, Cruz CF, Loureiro A, Nogueira P, Freitas J, Moreira A, Carmo AM, Gomes AC, Preto A, Cavaco-Paulo A
JournalBiochim Biophys Acta
PubMed ID26589183
'Efficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel ... More
IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation.
Authors
JournalCell Host Microbe
PubMed ID31175045
CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.
Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization.
Authors
JournalCell Host Microbe
PubMed ID33535027
Extrinsic Macrophages Protect While Tendon Progenitors Degrade: Insights from a Tissue Engineered Model of Tendon Compartmental Crosstalk.
Authors
JournalAdv Healthc Mater
PubMed ID34494401
A biomechanical switch regulates the transition towards homeostasis in oesophageal epithelium.
Authors
JournalNat Cell Biol
PubMed ID33972733
The Alzheimer's disease-associated C99 fragment of APP regulates cellular cholesterol trafficking.
Authors
JournalEMBO J
PubMed ID32865299
Differentiating responses of lung cancer cell lines to Doxorubicin exposure: in vitro Raman micro spectroscopy, oxidative stress and bcl-2 protein expression.
The potential of Raman micro spectroscopy as an in vitro, non-invasive tool for clinical applications has been demonstrated in recent years, specifically for cancer research. To further illustrate its potential as a high content and label free technique, it is important to show its capability to elucidate drug mechanisms of ... More
Concurrent live imaging of DNA double-strand break repair and cell-cycle progression by CRISPR/Cas9-mediated knock-in of a tricistronic vector.
AuthorsOtsuka K, Tomita M
JournalSci Rep
PubMed ID30470841
Cell-cycle progression can be arrested by ionizing radiation-induced DNA double-strand breaks (DSBs). Although DSBs are patched by DSB repair systems, which comprise proteins such as p53-binding protein 1 (53BP1), the relationship between DSB repair progression and cell-cycle status in living cells is unclear. The probe FUCCI (fluorescent ubiquitination-based cell-cycle indicator) ... More
Non-Thermal Plasma, as a New Physicochemical Source, to Induce Redox Imbalance and Subsequent Cell Death in Liver Cancer Cell Lines.
AuthorsSmolková B, Lunova M, Lynnyk A, Uzhytchak M, Churpita O, Jirsa M, Kubinová Š, Lunov O, Dejneka A
JournalCell Physiol Biochem
PubMed ID30790509
Alteration of cancer cell redox status has been recognized as a promising therapeutic implication. In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Thus, the aim of this ... More
Spatiotemporally Monitoring Cell Viability through Programmable Mitochondrial Membrane Potential Transformation by Using Fluorescent Carbon Dots.
AuthorsYin X, Sun Y, Geng X, Li J, Yang R, Zhang K, Qu L, Li Z
JournalAdv Biosyst
PubMed ID32293145
Mitochondria are the powerhouse of the cell, which maintain the metabolism of living cells. Mitochondrial membrane potential (MMP) is the key parameter of mitochondria and represents cellular activity. The programmable MMP transformations, i.e., normal, decrease, and vanishing, are indicative mitochondria healthy/damaged/dead states. Therefore, methods for monitoring MMP are of great ... More