'Human cytomegalovirus (CMV) is a latent and persistent virus whose proliferation increases morbidity and mortality of immune-compromised individuals. The current anti-CMV therapeutics targeting the viral DNA polymerase or the major immediate-early (MIE) gene locus are somewhat effective at limiting CMV-associated disease. However, due to low bioavailability, severe toxicity, and the ... More
Influenza A virus uses intercellular connections to spread to neighboring cells.
AuthorsRoberts KL, Manicassamy B, Lamb RA,
Journal
PubMed ID25428869
'In the extracellular environment, cell-free virions seek out naive host cells over long distances and between organisms. This is the primary mechanism of spread for most viruses. Here we provide evidence for an alternative pathway previously undescribed for orthomyxoviruses, whereby the spread of influenza A virus (IAV) infectious cores to ... More
Effects of macro- versus nanoporous silicon substrates on human aortic endothelial cell behavior.
AuthorsFormentín P, Alba M, Catalán U, Fernández-Castillejo S, Pallarès J, Solà R, Marsal LF,
Journal
PubMed ID25246859
Human aortic endothelial cells play a key role in the pathogenesis of atherosclerosis, which is a common, progressive, and multifactorial disease that is the clinical endpoint of an inflammatory process and endothelial dysfunction. Study and development of new therapies against cardiovascular disease must be tested in vitro cell models, prior ... More
Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury.
AuthorsRusso MV, Latour LL, McGavern DB,
JournalNat Immunol
PubMed ID29662169
'Mild traumatic brain injury (mTBI) can cause meningeal vascular injury and cell death that spreads into the brain parenchyma and triggers local inflammation and recruitment of peripheral immune cells. The factors that dictate meningeal recovery after mTBI are unknown at present. Here we demonstrated that most patients who had experienced ... More
Differentiation of ciliated human midbrain-derived LUHMES neurons.
Authors
JournalJ Cell Sci
PubMed ID34005365
Glycogen controls Caenorhabditis elegans lifespan and resistance to oxidative stress.
AuthorsGusarov I, Pani B, Gautier L, Smolentseva O, Eremina S, Shamovsky I, Katkova-Zhukotskaya O, Mironov A, Nudler E,
JournalNat Commun
PubMed ID28627510
'A high-sugar diet has been associated with reduced lifespan in organisms ranging from worms to mammals. However, the mechanisms underlying the harmful effects of glucose are poorly understood. Here we establish a causative relationship between endogenous glucose storage in the form of glycogen, resistance to oxidative stress and organismal aging ... More
Engineering butylglyceryl-modified polysaccharides towards nanomedicines for brain drug delivery.
AuthorsBostanudin MF, Lalatsa A, Górecki DC, Barbu E
JournalCarbohydr Polym
PubMed ID32172875
'Colloidal systems prepared from carbohydrates are subject of intense research due to their potential to enhance drug permeability through biological membranes, however their characteristics and performance are never compared directly. Here we report the results of a comparative investigation of a series of butylglyceryl-modified polysaccharides (chitosan, guar gum, and pullulan) ... More
Ultrathin Polymer Membranes with Patterned, Micrometric Pores for Organs-on-Chips.
Authors
JournalACS Appl Mater Interfaces
PubMed ID27513606
Synthesis, Physicochemical Characterization, and Cytotoxicity Assessment of Rh Nanoparticles with Different Morphologies-as Potential XFCT Nanoprobes.
Authors
JournalNanomaterials (Basel)
PubMed ID33120889
Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture.
Authors
JournalMicromachines (Basel)
PubMed ID33567528
Phthalimide Derivative Shows Anti-angiogenic Activity in a 3D Microfluidic Model and No Teratogenicity in Zebrafish Embryos.
Authors
JournalFront Pharmacol
PubMed ID31057399
Development and characterization of a microfluidic model of the tumour microenvironment.
Authors
JournalSci Rep
PubMed ID27796335
Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells.
Authors
JournalPolymers (Basel)
PubMed ID33143320
Neutrophil extracellular traps promote metastasis in gastric cancer patients with postoperative abdominal infectious complications.
Authors
JournalNat Commun
PubMed ID35197446
Differentiation of ciliated human midbrain-derived LUHMES neurons.
Authors
JournalJ Cell Sci
PubMed ID33115758
A new agarose-based microsystem to investigate cell response to prolonged confinement.
Authors
JournalLab Chip
PubMed ID32975276
Fluorous photosensitizers enhance photodynamic therapy with perfluorocarbon nanoemulsions.
AuthorsDay RA, Estabrook DA, Logan JK, Sletten EM
JournalChem Commun (Camb)
PubMed ID29164187
Photodynamic therapy (PDT) requires a photosensitizer, light, and oxygen to induce cell death. The majority of efforts to advance PDT focus only on the first two components. Here, we employ perfluorocarbon nanoemulsions to simultaneously deliver oxygen and a photosensitizer. We find that the implementation of fluorous soluble photosensitizers enhances the ... More
Differential modulation of Bax/Bcl-2 ratio and onset of caspase-3/7 activation induced by derivatives of Justicidin B in human melanoma cells A375.
AuthorsAl-Qathama A, Gibbons S, Prieto JM
JournalOncotarget
PubMed ID29221182
Diphyllin and its derivatives are well known cytotoxic natural products structurally related to the anti-cancer drug podophyllotoxin. We here study their structure-activity relationship upon human melanoma cells for first time. To this end, human melanoma A375 cells were incubated with Justicidin B and its 4-methoxylated or 4-glycosylated derivatives to evaluate ... More
Loss of ATM in Airway Epithelial Cells Is Associated with Susceptibility to Oxidative Stress.
Background: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to ... More
Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.
AuthorsYe J, Tang S, Meng L, Li X, Wen X, Chen S, Niu L, Li X, Qiu W, Hu H, Jiang M, Shang S, Shu Q, Zheng H, Duan S, Li Y,
JournalNano Lett
PubMed ID29916253
Externally controlling the excitation of a neuronal subset through ion channels activation can modulate the firing pattern of an entire neural circuit in vivo. As nanovalves in the cell membrane, ion channels can be opened by light (optogenetics) or ultrasonic (sonogenetics) means. A thoroughly analyzed force sensor is the Escherichia ... More
Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures.
AuthorsFormentín P, Catalán Ú, Pol L, Fernández-Castillejo S, Solà R, Marsal LF,
JournalJ Biol Eng
PubMed ID30305842
The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon ... More