Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials.
AuthorsLin ZC, Xie C, Osakada Y, Cui Y, Cui B,
Journal
PubMed ID24487777
'Intracellular recording of action potentials is important to understand electrically-excitable cells. Recently, vertical nanoelectrodes have been developed to achieve highly sensitive, minimally invasive and large-scale intracellular recording. It has been demonstrated that the vertical geometry is crucial for the enhanced signal detection. Here we develop nanoelectrodes of a new geometry, ... More
The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages.
AuthorsTweedell R, Tao D, Dinglasan RR,
Journal
PubMed ID25266554
Kupffer cells (KCs) are the first line of defense in the liver against pathogens, yet several microbes successfully target the liver, bypass immune surveillance, and effectively develop in this tissue. Our current, albeit poor, understanding of KC-pathogen interactions has been largely achieved through the study of primary cells, requiring isolation ... More
Distinct adipogenic differentiation phenotypes of human umbilical cord mesenchymal cells dependent on adipogenic conditions.
AuthorsSaben J, Thakali KM, Lindsey FE, Zhong Y, Badger TM, Andres A, Shankar K,
Journal
PubMed ID24951473
The umbilical cord (UC) matrix is a source of multipotent mesenchymal stem cells (MSCs) that have adipogenic potential and thus can be a model to study adipogenesis. However, existing variability in adipocytic differentiation outcomes may be due to discrepancies in methods utilized for adipogenic differentiation. Additionally, functional characterization of UCMSCs ... More
CD8 T Cells Use IFN-? To Protect against the Lethal Effects of a Respiratory Poxvirus Infection.
CD8 T cells are a key component of immunity to many viral infections. They achieve this through using an array of effector mechanisms, but precisely which component/s are required for protection against a respiratory orthopox virus infection remains unclear. Using a model of respiratory vaccinia virus infection in mice, we ... More
An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14.
AuthorsZhao N, Zhang AS, Worthen C, Knutson MD, Enns CA,
Journal
PubMed ID24927598
Protein degradation is instrumental in regulating cellular function. Plasma membrane proteins targeted for degradation are internalized and sorted to multivesicular bodies, which fuse with lysosomes, where they are degraded. ZIP14 is a newly identified iron transporter with multitransmembrane domains. In an attempt to dissect the molecular mechanisms by which iron ... More
Inhibition of CPAP-tubulin interaction prevents proliferation of centrosome-amplified cancer cells.
Authors
JournalEMBO J
PubMed ID30530478
Loss of tuberous sclerosis complex 2 sensitizes tumors to nelfinavir-bortezomib therapy to intensify endoplasmic reticulum stress-induced cell death.
Authors
JournalOncogene
PubMed ID29980790
Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples.
AuthorsMan T, Wan Y, Yan W, Wang XH, Peterman EJG, Wang D,
JournalBiomed Opt Express
PubMed ID30258677
'Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is ... More
Inhibition of the methyltranferase EZH2 improves aortic performance in experimental thoracic aortic aneurysm.
'Loss-of-function mutations in genes encoding contractile proteins have been observed in thoracic aortic aneurysms (TAA). To gain insight into the contribution of contractile protein deficiency in the pathogenesis of TAA, we examined human aneurysm samples. We found multiple contractile gene products deficient in TAA samples, and in particular, expression of ... More
Biomimetic mineralized strontium-doped hydroxyapatite on porous poly(l-lactic acid) scaffolds for bone defect repair.
AuthorsGe M, Ge K, Gao F, Yan W, Liu H, Xue L, Jin Y, Ma H, Zhang J,
JournalInt J Nanomedicine
PubMed ID29599615
'poly(l-lactic acid) (PLLA) has been approved for clinical use by the US Food and Drug Administration (FDA); however, their stronger hydrophobicity and relatively fast degradation rate restricted their widespread application. In consideration of the composition of bone, the inorganic-organic composite has a great application prospect in bone tissue engineering. Many ... More
Large-scale fabrication of free-standing and sub-µm PDMS through-hole membranes.
AuthorsLe-The H, Tibbe M, Loessberg-Zahl J, Palma do Carmo M, van der Helm M, Bomer J, van den Berg A, Leferink A, Segerink L, Eijkel J,
JournalNanoscale
PubMed ID29658030
'Free-standing polydimethylsiloxane (PDMS) through-hole membranes have been studied extensively in recent years for chemical and biomedical applications. However, robust fabrication of such membranes with sub-µm through-holes, and at a sub-µm thickness over large areas is challenging. In this paper, we report a robust and simple method for large-scale fabrication of ... More
AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption.
AuthorsYokoi A, Yoshioka Y, Yamamoto Y, Ishikawa M, Ikeda SI, Kato T, Kiyono T, Takeshita F, Kajiyama H, Kikkawa F, Ochiya T,
JournalNat Commun
PubMed ID28262727
'Advanced ovarian cancers are highly metastatic due to frequent peritoneal dissemination, resulting in dismal prognosis. Here we report the functions of cancer-derived extracellular vesicles (EVs), which are emerging as important mediators of tumour metastasis. The EVs from highly metastatic cells strongly induce metastatic behaviour in moderately metastatic tumours. Notably, the ... More
Overexpression of Insulin-Like Growth Factor 1 Enhanced the Osteogenic Capability of Aging Bone Marrow Mesenchymal Stem Cells.
AuthorsChen CY, Tseng KY, Lai YL, Chen YS, Lin FH, Lin S,
JournalTheranostics
PubMed ID28529639
'Many studies have indicated that loss of the osteoblastogenic potential in bone marrow mesenchymal stem cells (bmMSCs) is the major component in the etiology of the aging-related bone deficit. But how the bmMSCs lose osteogenic capability in aging is unclear. Using 2-dimentional cultures, we examined the dose response of human ... More
Therapeutic Targeting of Sunitinib-Induced AR Phosphorylation in Renal Cell Carcinoma.
Authors
JournalCancer Res
PubMed ID29572225
The use of 3D cultures of MCF-10A and MCF-12A cells by high content screening for effect-based analysis of non-genotoxic carcinogens.
AuthorsEngel A, Frenzel F, Niemann B, Braeuning A, Lampen A, Buhrke T
JournalToxicol In Vitro
PubMed ID30974152
'The human breast epithelial cell lines MCF-10A and MCF-12A form well-differentiated acinus-like structures when grown in three-dimensional matrigel culture over a period of 20?days. In the present study, both cell lines were tested for their suitability to serve as an effect-based in vitro test system for non-genotoxic carcinogens. A software ... More
A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling.
Authors
JournalCell Rep
PubMed ID32268091
CX3CR1 positively regulates BCR signaling coupled with cell metabolism via negatively controlling actin remodeling.
Authors
JournalCell Mol Life Sci
PubMed ID32016488
Brain metastatic cancer cells release microRNA-181c-containing extracellular vesicles capable of destructing blood-brain barrier.
Endothelial cells release ATP in response to fluid shear stress, which activates purinergic (P2) receptor-mediated signaling molecules including endothelial nitric oxide (eNOS), a regulator of vascular tone. While P2 receptor-mediated signaling in the vasculature is well studied, the role of P2Y ... More
Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway.
AuthorsTaverna S, Pucci M, Giallombardo M, Di Bella MA, Santarpia M, Reclusa P, Gil-Bazo I, Rolfo C, Alessandro R,
JournalSci Rep
PubMed ID28600504
Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related deaths worldwide. The majority of patients are diagnosed in advanced disease stage. Bone metastasis is the most frequent complication in NSCLC resulting in osteolytic lesions. The perfect balance between bone-resorbing osteoclasts and bone-forming osteoblasts activity is lost in bone ... More
Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing.
AuthorsGindele JA, Mang S, Pairet N, Christ I, Gantner F, Schymeinsky J, Lamb DJ,
JournalPLoS One
PubMed ID28863189
Inappropriate repair responses to pulmonary epithelial injury have been linked to perturbation of epithelial barrier function and airway remodelling in a number of respiratory diseases, including chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. We developed an in vitro mechanical scratch injury model in air-liquid interface differentiated primary human small ... More
A full lifespan model of vertebrate lens growth.
AuthorsŠikic H, Shi Y, Lubura S, Bassnett S,
JournalR Soc Open Sci
PubMed ID28280571
The mathematical determinants of vertebrate organ growth have yet to be elucidated fully. Here, we utilized empirical measurements and a dynamic branching process-based model to examine the growth of a simple organ system, the mouse lens, from E14.5 until the end of life. Our stochastic model used difference equations to ... More
Fabrication and characterization of cell sheets using methylcellulose and PNIPAAm thermoresponsive polymers: A comparison Study.
AuthorsForghani A, Kriegh L, Hogan K, Chen C, Brewer G, Tighe TB, Devireddy R, Hayes D,
JournalJ Biomed Mater Res A
PubMed ID28130868
Culturing cells on thermoresponsive polymers enables cells to be harvested as an intact cell sheet without disrupting the extracellular matrix or compromising cell-cell junctions. Previously, cell sheet fabrication methods using methylcellulose (MC) gel and PNIPAAm were independently demonstrated. In this study, MC and PNIPAAm fabrication methods are detailed and the ... More
A highly printable and biocompatible hydrogel composite for direct printing of soft and perfusable vasculature-like structures.
AuthorsSuntornnond R, Tan EYS, An J, Chua CK,
JournalSci Rep
PubMed ID29203812
Vascularization is one major obstacle in bioprinting and tissue engineering. In order to create thick tissues or organs that can function like original body parts, the presence of a perfusable vascular system is essential. However, it is challenging to bioprint a hydrogel-based three-dimensional vasculature-like structure in a single step. In ... More
Novel device for application of continuous mechanical tensile strain to mammalian cells.
AuthorsWada S, Kanzaki H, Narimiya T, Nakamura Y,
JournalBiol Open
PubMed ID28302667
During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we ... More
Development of noncytotoxic silver-chitosan nanocomposites for efficient control of biofilm forming microbes.
AuthorsRegiel-Futyra A, Kus-Liskiewicz M, Sebastian V, Irusta S, Arruebo M, Kyziol A, Stochel G,
JournalRSC Adv
PubMed ID29308194
Severe bacterial and fungal infections have become a major clinical and public health concern. Nowadays, additional efforts are needed to develop effective antimicrobial materials that are not harmful to human cells. This work describes the synthesis and characterization of chitosan-ascorbic acid-silver nanocomposites as films exhibiting high antimicrobial activity and non-cytotoxicity ... More
A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs.
AuthorsAthirasala A, Lins F, Tahayeri A, Hinds M, Smith AJ, Sedgley C, Ferracane J, Bertassoni LE,
JournalSci Rep
PubMed ID28607361
The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals ... More
The introduction of mesenchymal stromal cells induces different immunological responses in the lungs of healthy and M. tuberculosis infected mice.
AuthorsNenasheva T, Nikolaev A, Diykanov D, Sukhanova A, Tcyganov E, Panteleev A, Bocharova I, Serdyuk Y, Nezlin L, Radaeva T, Adrianov N, Rubtsov Y, Lyadova I,
JournalPLoS One
PubMed ID28594940
Mesenchymal stromal cells (MSC) have strong immunomodulatory properties and therefore can be used to control inflammation and tissue damage. It was suggested recently that MSC injections can be used to treat multi-drug resistant tuberculosis (TB). However, MSC trafficking and immunomodulatory effects of MSC injections during Mycobacterium tuberculosis (Mtb) infection have ... More
AuthorsRobinson E, MacDonald KD, Slaughter K, McKinney M, Patel S, Sun C, Sahay G,
JournalMol Ther
PubMed ID29910178
The promise of gene therapy for the treatment of cystic fibrosis has yet to be fully clinically realized despite years of effort toward correcting the underlying genetic defect in the cystic fibrosis transmembrane conductance regulator (CFTR). mRNA therapy via nanoparticle delivery represents a powerful technology for the transfer of genetic material ... More
Transgelin induces dysfunction of fetal endothelial colony-forming cells from gestational diabetic pregnancies.
AuthorsVarberg KM, Garretson RO, Blue EK, Chu C, Gohn CR, Tu W, Haneline LS,
JournalAm J Physiol Cell Physiol
PubMed ID29949406
Fetal exposure to gestational diabetes mellitus (GDM) predisposes children to future health complications including hypertension and cardiovascular disease. A key mechanism by which these complications occur is through the functional impairment of vascular progenitor cells, including endothelial colony-forming cells (ECFCs). Previously, we showed that fetal ECFCs exposed to GDM have ... More
Biocompatibility of highly purified bovine milk-derived extracellular vesicles.
AuthorsSomiya M, Yoshioka Y, Ochiya T,
JournalJ Extracell Vesicles
PubMed ID29511463
Extracellular vesicles (EVs) deliver biologically active cargos from donor cells to recipient cells for intercellular communication. Since the existence of RNA cargo was discovered, EVs have been considered to be useful drug-delivery systems. Specifically, EVs from bovine milk (mEV) are one of the most promising platforms, since bovine milk is ... More
Localization of RNA and translation in the mammalian oocyte and embryo.
AuthorsJansova D, Tetkova A, Koncicka M, Kubelka M, Susor A,
JournalPLoS One
PubMed ID29529035
The tight correlation between mRNA distribution and subsequent protein localization and function indicate a major role for mRNA localization within the cell. RNA localization, followed by local translation, presents a mechanism for spatial and temporal gene expression regulation utilized by various cell types. However, little is known about mRNA localization ... More
Differentiation of human iPSCs into functional podocytes.
AuthorsRauch C, Feifel E, Kern G, Murphy C, Meier F, Parson W, Beilmann M, Jennings P, Gstraunthaler G, Wilmes A,
JournalPLoS One
PubMed ID30222766
Podocytes play a critical role in glomerular barrier function, both in health and disease. However, in vivo terminally differentiated podocytes are difficult to be maintained in in vitro culture. Induced pluripotent stem cells (iPSCs) offer the unique possibility for directed differentiation into mature podocytes. The current differentiation protocol to generate ... More
A new bioluminescence-based tool for modulating target proteins in live cells.
AuthorsIshimoto T, Mori H
JournalSci Rep
PubMed ID31796796
We have developed a new genetically encoded tool designed to generate reactive oxygen species (ROS) at target proteins in cultured cells; it is designed using firefly luciferase and photosensitiser protein KillerRed. Targeting this fusion protein, KillerFirefly, to F-actin in live cells and treatment with luciferin induced a characteristic structure, previously ... More
Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy.
AuthorsIshida T, Fujihara N, Nishimura T, Funabashi H, Hirota R, Ikeda T, Kuroda A
JournalGenes Environ
PubMed ID31178942
Frustrated phagocytosis occurs when an asbestos fiber >?10?µm in length is engulfed imperfectly by a macrophage, and it is believed to be associated with chromosomal instability. Few studies have focused on dynamic cellular imaging to assess the toxicity of hazardous inorganic materials such as asbestos. One reason for this is ... More
The Absence of Tryptase Mcpt6 Causes Elevated Cellular Stress in Response to Modulation of the Histone Acetylation Status in Mast Cells.
AuthorsSantosh Martin S, Rabelo Melo F, Pejler G
JournalCells
PubMed ID31581668
Mast cells contain large amounts of proteases stored within their secretory granules. Previously we showed that one of these proteases, tryptase, in addition to its location within granules, can also be found within the mast cell nucleus, where it has the capacity to affect the acetylation profile of nucleosomal core ... More
Neuroligin-3 and Neuroligin-4X form Nanoscopic clusters and regulate growth cone organization and size.
The cell-adhesion proteins neuroligin-3 and neuroligin-4X (NLGN3/4X) have well described roles in synapse formation. NLGN3/4X are also expressed highly during neurodevelopment. However, the role these proteins play during this period is unknown. Here we show that NLGN3/4X localized to the leading edge of growth cones where it promoted neuritogenesis in ... More