细胞成像应用 CellEvent™ 半胱天冬酶-3/7 Green 试剂是一种与核酸结合染料偶联的四氨基酸肽 (DEVD),其未与 DNA 结合时不发出荧光。CellEvent™ 半胱天冬酶-3/7 Green 试剂本质上不发出荧光,因为 DEVD 多肽可抑制染料与 DNA 的结合。凋亡细胞中半胱天冬酶-3/7 活化后,DEVD 肽被切割,游离的染料可与 DNA 结合,从而产生明亮的绿色荧光。当染料与 DNA 结合时,在 530 nm 处有荧光发射,可以使用标准 FITC 滤光片组观察该荧光发射情况。
I want to use your CellEvent Caspase-3/7 Green detection reagent for studying apoptosis in live cells, but for 96-well microplate instead of microscopy. Will it work? Can I multiplex it with a cell vitality dye?
Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.