Ammonia excretion in the Atlantic hagfish (Myxine glutinosa) and responses of an Rhc glycoprotein.
AuthorsEdwards SL, Arnold JM, Blair SD, Pray ME, Bradley RR, Erikson O, Walsh PJ,
Journal
PubMed ID25715835
Hagfishes are the most ancient of the extant craniates, and demonstrate a high tolerance for a number of unfavorable environmental conditions including elevated ammonia. Proposed mechanisms of ammonia excretion in aquatic organisms include vesicular NH4+ transport and release by exocytosis in marine crabs, and passive NH3 diffusion, active NH4+ transport ... More
ALS-associated FUS mutations result in compromised FUS alternative splicing and autoregulation.
AuthorsZhou Y, Liu S, Liu G, Oztürk A, Hicks GG,
Journal
PubMed ID24204307
The gene encoding a DNA/RNA binding protein FUS/TLS is frequently mutated in amyotrophic lateral sclerosis (ALS). Mutations commonly affect its carboxy-terminal nuclear localization signal, resulting in varying deficiencies of FUS nuclear localization and abnormal cytoplasmic accumulation. Increasing evidence suggests deficiencies in FUS nuclear function may contribute to neuron degeneration. Here ... More
Hepatic DNA deposition drives drug-induced liver injury and inflammation in mice.
AuthorsMarques PE, Oliveira AG, Pereira RV, David BA, Gomides LF, Saraiva AM, Pires DA, Novaes JT, Patricio DO, Cisalpino D, Menezes-Garcia Z, Leevy WM, Chapman SE, Mahecha G, Marques RE, Guabiraba R, Martins VP, Souza DG, Mansur DS, Teixeira MM, Leite MF, Menezes GB,
Journal
PubMed ID24824608
Drug-induced liver injury (DILI) is an important cause of acute liver failure, with limited therapeutic options. During DILI, oncotic necrosis with concomitant release and recognition of intracellular content amplifies liver inflammation and injury. Among these molecules, self-DNA has been widely shown to trigger inflammatory and autoimmune diseases; however, whether DNA ... More
A map of gene expression in neutrophil-like cell lines.
AuthorsRincón E, Rocha-Gregg BL, Collins SR,
JournalBMC Genomics
PubMed ID30068296
'Human neutrophils are central players in innate immunity, a major component of inflammatory responses, and a leading model for cell motility and chemotaxis. However, primary neutrophils are short-lived, limiting their experimental usefulness in the laboratory. Thus, human myeloid cell lines have been characterized for their ability to undergo neutrophil-like differentiation ... More
Therapeutic Targeting of PTK7 is Cytotoxic in Atypical Teratoid Rhabdoid Tumors.
AuthorsMesserli SM, Hoffman MM, Gnimpieba EZ, Bhardwaj RD,
JournalMol Cancer Res
PubMed ID28442586
'Novel discoveries involving the evaluation of potential therapeutics are based on newly identified molecular targets for atypical teratoid rhabdoid tumors (ATRT), which are the most common form of infantile brain tumors. Central nervous system ATRTs are rare, aggressive, and fast growing tumors of the brain and spinal cord and carry ... More
Therapy response testing of breast cancer in a 3D high-throughput perfused microfluidic platform.
AuthorsLanz HL, Saleh A, Kramer B, Cairns J, Ng CP, Yu J, Trietsch SJ, Hankemeier T, Joore J, Vulto P, Weinshilboum R, Wang L,
JournalBMC Cancer
PubMed ID29096610
'Breast cancer is the most common invasive cancer among women. Currently, there are only a few models used for therapy selection, and they are often poor predictors of therapeutic response or take months to set up and assay. In this report, we introduce a microfluidic OrganoPlate® platform for extracellular matrix ... More
Guanine quadruplex monoclonal antibody 1H6 cross-reacts with restrained thymidine-rich single stranded DNA.
AuthorsKazemier HG, Paeschke K, Lansdorp PM,
JournalNucleic Acids Res
PubMed ID28449085
'Previously we reported the production and characterization of monoclonal antibody 1H6 raised against (T4G4)2 intermolecular guanine quadruplex (G4) DNA structures (Henderson A. et al. (2014) Nucleic Acids Res., 42, 860-869; Hoffmann R.F. et al. (2016) Nucleic Acids Res., 44, 152-163). It was shown that 1H6 strongly stains nuclei and has exquisite specificity ... More
Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay.
AuthorsOsorio JS, Bionaz M,
JournalGene
PubMed ID28501631
'Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of transcription factors, which are central to the study of nutrigenomics. However, this technology relies heavily on the insertion of foreign plasmid DNA into the nuclei of cells (i.e., transfection), which can be very ... More
Induced prodrug activation by conditional protein degradation.
AuthorsGaynor AS, Chen W,
JournalJ Biotechnol
PubMed ID28912080
'Enzyme prodrug therapies hold potential as a targeted treatment option for cancer patients. However, off-target effects can be detrimental to patient health and represent a safety concern. This concern can be alleviated by including a failsafe mechanism that can abort the therapy in healthy cells. This feature can be included ... More
Pathophysiological role of microRNA-29 in pancreatic cancer stroma.
Authors
JournalSci Rep
PubMed ID26095125
Mechanical competition triggered by innate immune signaling drives the collective extrusion of bacterially infected epithelial cells.
High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells.
Authors
JournalNat Commun
PubMed ID35697674
CD8+ T cells inhibit metastasis and CXCL4 regulates its function.
Authors
JournalBr J Cancer
PubMed ID33795809
Capacity of Pneumococci to Activate Macrophage Nuclear Factor κB: Influence on Necroptosis and Pneumonia Severity.
Authors
JournalJ Infect Dis
PubMed ID28368460
Behavioral and neural network abnormalities in human APP transgenic mice resemble those of App knock-in mice and are modulated by familial Alzheimer's disease mutations but not by inhibition of BACE1.
Authors
JournalMol Neurodegener
PubMed ID32921309
Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.
Authors
JournalNat Cell Biol
PubMed ID32839551
Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.
Authors
JournalCell Chem Biol
PubMed ID32698018
NEUROG1 Regulates CDK2 to Promote Proliferation in Otic Progenitors.
AuthorsSong Z, Jadali A, Fritzsch B, Kwan KY
JournalStem Cell Reports
PubMed ID29033307
Loss of spiral ganglion neurons (SGNs) significantly contributes to hearing loss. Otic progenitor cell transplantation is a potential strategy to replace lost SGNs. Understanding how key transcription factors promote SGN differentiation in otic progenitors accelerates efforts for replacement therapies. A pro-neural transcription factor, Neurogenin1 (Neurog1), is essential for SGN development. ... More
Unique spectral signatures of the nucleic acid dye acridine orange can distinguish cell death by apoptosis and necroptosis.
Cellular injury and death are ubiquitous features of disease, yet tools to detect them are limited and insensitive to subtle pathological changes. Acridine orange (AO), a nucleic acid dye with unique spectral properties, enables real-time measurement of RNA and DNA as proxies for cell viability during exposure to various noxious ... More
Inhibition of apoptosis exacerbates fatigue-damage tendon injuries in an in vivo rat model.
AuthorsBell R, Robles-Harris MA, Anderson M, Laudier D, Schaffler MB, Flatow EL, Andarawis-Puri N,
JournalEur Cell Mater
PubMed ID30058060
Tendinopathy is a common and progressive musculoskeletal disease. Increased apoptosis is an end-stage tendinopathy manifestation, but its contribution to the pathology of the disease is unknown. A previously established in vivo model of fatigue damage accumulation shows that increased apoptosis is correlated with the severity of induced tendon damage, even ... More
Tracking of quiescence in Leishmania by quantifying the expression of GFP in the ribosomal DNA locus.
AuthorsJara M, Maes I, Imamura H, Domagalska MA, Dujardin JC, Arevalo J
JournalSci Rep
PubMed ID31831818
Under stressful conditions some microorganisms adopt a quiescent stage characterized by a reversible non or slow proliferative condition that allows their survival. This adaptation was only recently discovered in Leishmania. We developed an in vitro model and a biosensor to track quiescence at population and single cell levels. The biosensor ... More