SYTOX™ Green Ready Flow™ 试剂
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SYTOX™ Green Ready Flow™ 试剂
Invitrogen™

SYTOX™ Green Ready Flow™ 试剂

SYTOX™ Green Ready Flow™ 试剂是一种明亮、易于使用的核酸染料，用于在流式细胞术中区分活细胞。激发波长为 488 nm 时，其发射光谱类似于了解更多信息
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货号数量
R37168120 次检测
货号 R37168
价格（CNY)
2,688.00
飞享价
Ends: 31-Dec-2025
3,571.00
共减 883.00 (25%)
Each
添加至购物车
数量:
120 次检测
价格（CNY)
2,688.00
飞享价
Ends: 31-Dec-2025
3,571.00
共减 883.00 (25%)
Each
添加至购物车
SYTOX™ Green Ready Flow™ 试剂是一种明亮、易于使用的核酸染料，用于在流式细胞术中区分活细胞。激发波长为 488 nm 时，其发射光谱类似于 FITC，这已在多种细胞系中经过测试。

•可在室温下保持稳定
• 方便使用的即用型溶液—无需稀释、称重或者移取
• 与核酸结合后发出明亮的绿色荧光
• 无需洗涤即可快速染色

SYTOX Green Ready Flow 试剂是一种即用型溶液，可直接对死细胞染色。直接向 1 x 106 个细胞中滴加 2 滴试剂，然后孵育、分析。

SYTOX Green 是一种不透膜染料，只有在膜不完整的情况下，才能进入细胞并与 DNA 结合，因此，作为复染剂时，可在 488 nm 处激发、在 523 nm 峰处捕获的发射波长进行分析。SYTOX Green 试剂可用于哺乳动物和细菌细胞，通常在多重检测中使用。

规格
细胞渗透性不透过
描述SYTOX™ Green Ready Flow™ 试剂
检测方法荧光
染料类型SYTOX™ Green Ready Flow™
形式溶液
产品规格瓶
数量120 次检测
溶解度DMSO（二甲亚砜）
颜色绿色
发射523 nm
Excitation Wavelength Range504 nm
适用于（应用）活力测定试剂盒
适用于（设备）流式细胞仪
产品线Ready Flow™，Sytox™
产品类型试剂名称
Unit SizeEach
内容与储存
包含2个滴瓶，在室温下储存。

常见问题解答 (FAQ)

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.